A competitive ELISA (ELISA) for the quantitation of 3,5,6-trichloro-2-pyridinol (TCP), the major biol. and environmental degradation product of chlorpyrifos and triclopyr, was developed.Magnetic particles were used as the solid phase to attach monoclonal anti-TCP antibodies.The ELISA has an estimated least detectable dose of 0.25 ppb in water.Specificity studies indicate that the assay is specific for TCP and can distinguish it from the parent compounds as well as many other related and unrelated agricultural compoundsResults compare favorably with GC/MSD measurements (r = 0.959).

23 Oct 1996·ACS Symposium Series

Extracts of Fish and Dialysates of Semipermeable Membrane Devices

Author: Huckins, James N. ; Tillitt, Donald E. ; Nardone, David A. ; Potts, Michael E. ; Petty, Jimmie D. ; Zajicek, James L.

23 Oct 1996·ACS Symposium Series

Considerations in Immunoassay Calibration

Author: Fare, Thomas L. ; Sandberg, Robert G. ; Herzog, David P.

A review, with 8 references, is given on calibration of small mol. immunoassays for quant. analyses.The appeal of immunoassays was their ability to provide precise and accurate quant. results for a specific analyte at a low cost per test.This quant. capability was the motivation for anal. chemists to evaluate the technique for use with environmental samples.While some regulators have initially viewed immunoassays as a qual. method, more recently they have expressed interest in extending the application of immunoassay beyond screening results to quant. analyses.Although many of the principles underlying qual. and quant. techniques are similar, qual. evaluation of data, particularly when applied to the detection of complex mixtures, is sufficiently complex to deserve sep. treatment.A general approach to important considerations will be given along with a set of recommendations for the analyst using immunochem. methods.

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