The objective of the paper is to determine the ginsenoside Rb1 content in Radix et Rhizoma notoginseng in Wenxin Capsule.The HPLC method was developed for the quant. anal. of ginsenoside Rb1.The separation was performed on the column of C18 (150 mm × 4.6 mm, 5 μm).The mobile phase was acetonitrile-0.1% phosphoric acid solution (30:70), the detection wavelength was 203 nm.The results showed that the linear range of ginsenoside Rb1 was in 30.6-153 μg/mL (r = 0.999 7), the average recovery rate was 99.31%, RSD was 0.34% (n = 6).It was concluded that the method is accurate, simple, and fast.It can be used for the content determination and the quality control of ginsenoside Rb1 in Radix et Rhizoma notoginseng in Wenxin Capsule.