CCZ1

Last update 08 May 2025

Basic Info

Synonyms

C7orf28A, CCZ1, CCZ1 homolog, vacuolar protein trafficking and biogenesis associated

+ [3]

Introduction

Acts in concert with MON1A, as a guanine exchange factor (GEF) for RAB7, promotes the exchange of GDP to GTP, converting it from an inactive GDP-bound form into an active GTP-bound form (PubMed:23084991).

100 Clinical Results associated with CCZ1

100 Translational Medicine associated with CCZ1

0 Patents (Medical) associated with CCZ1

Literatures (Medical) associated with CCZ1

01 Feb 2025·Plant, Cell & Environment

Tissue‐Specific Regulation of Vesicular Trafficking Mediated by Rab‐GEF Complex MON1/CCZ1 From Solanum chilense Increases Salt Stress Tolerance in Arabidopsis thaliana

Article

Author: Norambuena, Lorena ; Ruiz‐Lara, Simón ; Madrid‐Espinoza, José ; Salinas‐Cornejo, Josselyn

ABSTRACTSalt stress constrains the development and growth of plants. To tolerate it, mechanisms of endocytosis and vacuolar compartmentalization of Na+ are induced. In this work, the genes that encode a putative activator of vesicular trafficking called MON1/CCZ1 from Solanum chilense, SchMON1 and SchCCZ1, were co‐expressed in roots of Arabidopsis thaliana to determine whether the increase in prevacuolar vesicular trafficking also increases the Na+ compartmentalization capacity and tolerance. Initially, we demonstrated that both SchMON1 and SchCCZ1 genes rescued the dwarf phenotype of both A. thaliana mon1‐1 and ccz1a/b mutants associated with the loss of function, and both proteins colocalized with their functional targets, RabF and RabG, in endosomes. Transgenic A. thaliana plants co‐expressing these genes improved salt stress tolerance compared to wild type plants, with SchMON1 contributing the most. At the sub‐cellular level, co‐expression of SchMON1/SchCCZ1 reduced ROS levels and increased endocytic activity, and number of acidic structures associated with autophagosomes. Notably, greater Na+ accumulation in vacuoles of cortex and endodermis was evidenced in the SchMON1 genotype. Molecular analysis of gene expression in each genotype supported these results. Altogether, our analysis shows that root activation of prevacuolar vesicular trafficking mediated by MON1/CCZ1 emerges as a promising physiological molecular mechanism to increase tolerance to salt stress in crops of economic interest.

02 Jan 2025·Autophagy

GORASP2 promotes phagophore closure and autophagosome maturation into autolysosomes

Article

Author: Zhang, Xing ; Zhao, Xiaodan ; Wang, Yijie ; Zhang, Zhenqian ; Zhang, Yue ; Huang, Lei ; Fu, Tingting ; Zhang, Xiaoyan ; Jian, Yannan ; Xing, Yusheng

As the central hub of the secretory pathway, the Golgi apparatus plays a crucial role in maintaining cellular homeostasis in response to stresses. Recent studies have revealed the involvement of the Golgi tether, GORASP2, in facilitating autophagosome-lysosome fusion by connecting LC3-II and LAMP2 during nutrient starvation. However, the precise mechanism remains elusive. In this study, utilizing super-resolution microscopy, we observed GORASP2 localization on the surface of autophagosomes during glucose starvation. Depletion of GORASP2 hindered phagophore closure by regulating the association between VPS4A and the ESCRT-III component, CHMP2A. Furthermore, we found that GORASP2 controls RAB7A activity by modulating its GEF complex, MON1A-CCZ1, thereby impacting RAB7A's interaction with the HOPS complex. The assembly of both STX17-SNAP29-VAMP8 and YKT6-SNAP29-STX7 SNARE complexes was also attenuated without GORASP2. These findings suggest that GORASP2 helps seal autophagosomes and activate the RAB7A-HOPS-SNAREs membrane fusion machinery for autophagosome maturation, highlighting its membrane tethering function in response to stresses.Abbreviations: BafA1: bafilomycin A₁; ESCRT: endosomal sorting complex required for transport; FPP: fluorescence protease protection; GEF: guanine nucleotide exchange factor; GFP: green fluorescent protein; GORASP2: golgi reassembly stacking protein 2; GSB: glucose starvation along with bafilomycin A₁; HOPS: homotypic fusion and protein sorting; LAMP2: lysosomal associated membrane protein 2; MAP1LC3B: microtubule associated protein 1 light chain 3 beta; PBS: phosphate-buffered saline; PtdIns3K: phosphatidylinositol 3-kinase; PtdIns3P: phosphatidylinositol-3-phosphate; PK: proteinase K; SNARE: soluble N-ethylmaleimide-sensitive factor attachment protein receptor; SIM: structured illumination microscopy; UVRAG: UV radiation resistance associated.

01 Jan 2025·Journal of Integrative Plant Biology

The MON1–CCZ1 complex plays dual roles in autophagic degradation and vacuolar protein transport in rice

Article

Author: Zhang, Yu ; Han, Xiaohang ; Wang, Fan ; Yan, Mengyuan ; Jing, Ruonan ; Jiang, Ling ; Zhang, Pengcheng ; Wan, Gexing ; Pan, Tian ; Chen, Yu ; Bao, Yiqun ; Ren, Yulong ; Zhao, Zhichao ; Zhu, Yun ; Zhang, Binglei ; Wu, Hongming ; Duan, Erchao ; Wan, Jianmin ; Wang, Yongfei ; Wang, Yihua ; Lei, Cailin ; Wang, Xin ; Bao, Xiuhao ; Sun, Xiejun ; Yan, Haigang ; Cheng, Zhijun

ABSTRACTAutophagy is a highly conserved cellular program in eukaryotic cells which mediates the degradation of cytoplasmic components through the lysosome, also named the vacuole in plants. However, the molecular mechanisms underlying the fusion of autophagosomes with the vacuole remain unclear. Here, we report the functional characterization of a rice (Oryza sativa) mutant with defects in storage protein transport in endosperm cells and accumulation of numerous autophagosomes in root cells. Cytological and immunocytochemical experiments showed that this mutant exhibits a defect in the fusion between autophagosomes and vacuoles. The mutant harbors a loss‐of‐function mutation in the rice homolog of Arabidopsis thaliana MONENSIN SENSITIVITY1 (MON1). Biochemical and genetic evidence revealed a synergistic interaction between rice MON1 and AUTOPHAGY‐RELATED 8a in maintaining normal growth and development. In addition, the rice mon1 mutant disrupted storage protein sorting to protein storage vacuoles. Furthermore, quantitative proteomics verified that the loss of MON1 function influenced diverse biological pathways including autophagy and vacuolar transport, thus decreasing the transport of autophagic and vacuolar cargoes to vacuoles. Together, our findings establish a molecular link between autophagy and vacuolar protein transport, and offer insights into the dual functions of the MON1–CCZ1 (CAFFEINE ZINC SENSITIVITY1) complex in plants.

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CCZ1

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