DNASE1L2

Last update 08 May 2025

Basic Info

Synonyms

deoxyribonuclease 1 like 2, Deoxyribonuclease I-like 2, Deoxyribonuclease-1-like 2

+ [5]

Introduction

Divalent cation-dependent acid DNA endonuclease involved in the breakdown of the nucleus during corneocyte formation of epidermal keratinocytes. May play an immune role by eliminating harmful DNA released into the extracellular environment by damaged epidermal cells.

100 Clinical Results associated with DNASE1L2

100 Translational Medicine associated with DNASE1L2

0 Patents (Medical) associated with DNASE1L2

Literatures (Medical) associated with DNASE1L2

01 Apr 2025·RNA

Schizosaccharomyces pombe pus1mutants are temperature sensitive due to decay of tRNA^Ile(UAU)by the 5′-3′ exonuclease Dhp1, primarily targeting the unspliced pre-tRNA

Article

Author: Phizicky, Eric M ; Mathews, David H ; Marcus, Erin ; Stegemann, Franziska ; Neupert, Savanah ; Ostrowski, Sarah

The pseudouridylase Pus1 catalyzes pseudouridine (Ψ) formation at multiple uridine residues in tRNAs, and in some snRNAs and mRNAs. Although Pus1 is highly conserved, and mutations are associated with human disease, little is known about eukaryotic Pus1 biology. Here, we show thatSchizosaccharomyces pombe pus1Δ mutants are temperature sensitive due to decay of tRNAIle(UAU), as tRNAIle(UAU)levels are reduced, and its overexpression suppresses the defect. We show that tRNAIle(UAU)is degraded by the 5′-3′ exonuclease Dhp1 (ortholog ofSaccharomyces cerevisiaeRat1), as each of four spontaneouspus1Δ suppressors haddhp1mutations and restored tRNAIle(UAU)levels, and two suppressors that also restored tRNAIle(UAU)levels had mutations intol1(S. cerevisiae MET22ortholog), predicted to inhibit Dhp1. We show that Pus1 modifies U27, U34, and U36of tRNAIle(UAU), raising the question about how these modifications prevent decay. Our results suggest that Dhp1 targets unspliced pre-tRNAIle(UAU), as apus1Δ strain in which the only copy of tRNAIle(UAU)has no intron [tI(UAU)-iΔ] is temperature resistant and undergoes no detectable decay, and the correspondingpus1ΔtI(UAU)-WTstrain accumulates unspliced pre-tRNAIle(UAU). Moreover, the predicted exon–intron structure of pre-tRNAIle(UAU)differs from the canonical bulge–helix–loop structure compatible with tRNA splicing, and apus1ΔtI(UAU)i-varstrain with intron mutations predicted to improve exon–intron structure is temperature resistant and undergoes little decay. These results suggest that decay of tRNAIle(UAU)by Dhp1 inpus1Δ strains occurs at the level of unspliced pre-tRNAIle(UAU), implying a substantial role for one or more of the Ψ residues in stabilizing the pre-tRNA structure for splicing.

01 Mar 2024·Cell Reports

RNAP II antagonizes mitotic chromatin folding and chromosome segregation by condensin

Article

Author: Colin, Léonard ; Bernard, Pascal ; Lebreton, Jérémy ; Chatre, Elodie

Condensin shapes mitotic chromosomes by folding chromatin into loops, but whether it does so by DNA-loop extrusion remains speculative. Although loop-extruding cohesin is stalled by transcription, the impact of transcription on condensin, which is enriched at highly expressed genes in many species, remains unclear. Using degrons of Rpb1 or the torpedo nuclease Dhp1^XRN2 to either deplete or displace RNAPII on chromatin in fission yeast metaphase cells, we show that RNAPII does not load condensin on DNA. Instead, RNAPII retains condensin in cis and hinders its ability to fold mitotic chromatin and to support chromosome segregation, consistent with the stalling of a loop extruder. Transcription termination by Dhp1 limits such a hindrance. Our results shed light on the integrated functioning of condensin, and we argue that a tight control of transcription underlies mitotic chromosome assembly by loop-extruding condensin.

01 Feb 2024·Bioelectrochemistry

Dumbbell hybridization chain reaction coupled with positively charged Au@luminol nanoparticles for enhanced electrochemiluminescent sensing of exosomal miRNA-21

Review

Author: Peng, Chenxu ; Miao, Xiangmin ; Li, Shiqiang ; Zhang, Yun ; Shi, Zhe

MicroRNAs (miRNAs) are important cancer biomarkers in cancer cell-derived exosomes. Herein, positively charged Au@luminol nanoparticles ((+)Au@luminol NPs) with enhanced electrochemiluminescence (ECL) and extreme stability were firstly established for the sensitive detection of miRNA-21 in exosome. In the presence of miRNA-21, dumbbell hybridization chain reaction (DHCR) happened at gold nanoparticles and ZIF-67 metal-organic framework modified glass carbon electrode (AuNP/ZIF-MOF/GCE) with the help of dumbbell DNA fuel strands (DHP1 and DHP2). The formed DHCR polymers were negatively charged and could electrostatically adsorb numbers of (+)Au@luminol NPs to produce strong ECL signal. Combing DHCR signal amplification with (+)Au@luminol NPs enhancer, sensitive detection of miRNA-21 realized with a detection limit of 0.43 fM. Moreover, the proposed method was successfully applied for the analysis of miRNA-21 in serum samples of healthy individuals and breast cancer patients, indicating a potential application value in early clinical diagnosis.

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DNASE1L2

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