CPSF4

Last update 08 May 2025

Basic Info

Synonyms

cleavage and polyadenylation specific factor 4, Cleavage and polyadenylation specificity factor 30 kDa subunit, Cleavage and polyadenylation specificity factor subunit 4

+ [8]

Introduction

Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. CPSF4 binds RNA polymers with a preference for poly(U).

100 Clinical Results associated with CPSF4

100 Translational Medicine associated with CPSF4

0 Patents (Medical) associated with CPSF4

728

Literatures (Medical) associated with CPSF4

01 Aug 2025·Water Research

Targeting regulation of nitrate removal and chlorophenol degradation through hydrogen/oxygen switching

Article

Author: Lu, Hongfeng ; Zhang, Ruichang ; Wei, Xuefeng ; Zhang, Ning ; Zhu, Shufa ; Ji, Jiantao ; Miao, Juan

Nitrate is a common co-contaminant with 2,4-dichlorophenol (2,4-DCP) in water, presenting a challenge for environmental remediation. Under anaerobic conditions, the ring cleavage of chlorophenol is inefficient, while under aerobic conditions, nitrate removal is hindered. In this study, a microbial consortium capable of hydrogenotrophic denitrification and 2,4-DCP degradation was cultured, aiming to achieve efficient nitrate removal and 2,4-DCP degradation by alternately switching between hydrogen (H₂) and oxygen (O₂). Under H₂ conditions, nitrate removal exceeded 90 %, while under O₂ conditions, 2,4-DCP degradation reached 100 %. Under H₂ conditions, the abundance of the Nar gene which was involved in nitrate reduction was higher than that under O₂ conditions, promoting hydrogenotrophic denitrification. In contrast, under O₂ conditions, 2,4-DCP degradation occurred via hydroxylation, ring-cleavage, dechlorination, and mineralization through the TCA cycle. Metagenomic and metabolomic analysis was performed to explore microbial metabolic pathways and potential synergistic mechanisms involved in hydrogenotrophic denitrification and 2,4-DCP biodegradation. In the H₂-atmosphere, microbes (Methylobacillus and Chromobacterium), genes (E3.1.1.45 and speG), and metabolites (Cytosine and Uridine) may play a crucial role in hydrogenotrophic denitrification. In the O₂-atmosphere, the functional genus of Paracoccus and Aquamicrobium associated with genes (tfdB and tfdC) may contribute to 2,4-DCP and its metabolites 2-Chloromaleylacetate degradation. These findings confirmed the role of functional microbial communities through H₂/O₂ regulation. This work provides a promising technological reference for treating industrial wastewater containing phenols and nitrogen.

01 Aug 2025·Water Research

Genome-resolved metagenomic analysis reveals a novel denitrifier with truncated nitrite reduction pathway from the genus SC-I-84

Article

Author: Lin, Yanting ; Meng, Fangang ; Chen, Qianqian ; Li, Jiapeng ; Zuo, Xiaotian

Understanding the genomic and ecological traits of partial denitrification (PD) bacteria is of high importance for developing wastewater treatment technologies. In this study, a PD-based bioreactor was operated, resulting in a mixed culture dominated by a potentially novel PD functional bacterium (SC-I-84). Progressively increased activity in both nitrate reduction and nitrite production were observed in the SC-I-84 enrichment system, whereas the nitrite reduction activity was always negligible. The phylogenetic analysis indicated that SC-I-84 was closely related to an uncultured beta-proteobacterium (99 %), whereas its denitrification functional genes (napA, napB, narV, and narY) exhibited evidence of co-evolution with chromosomal genes from the genus Cupriavidus, order Burkholderiales. In the genetic sketch of SC-I-84, only nitrate-reduction genes (nar and nap) were identified, whereas nitrite-reduction genes (nir) were absent. Notably, nitrate reduction genes were adjacent to carbon metabolism genes (sucB/C, mdh, idh) and a high abundance of tricarboxylic acid (TCA) cycling genes were found. This can promote the utilization efficiency of electron donors by nitrate reduction genes in SC-I-84, thus enhancing the denitrification activity. Furthermore, SC-I-84 positively cooperated with some bacteria that participate in nitrogen and carbon metabolism and other PD bacteria, but negatively interacted with full-denitrification bacteria. These results indicate that the enrichment of SC-I-84 restricted the growth of full-denitrification bacteria, aiding in the maintenance of a stable PD process. Taken together, the meta-genomic analysis of the novel PD functional bacterium is expected to enhance our understanding of PD processes and aid in the development of PD-based wastewater treatment processes.

01 Jul 2025·Journal of Hazardous Materials

Comprehensive revealing the destructive effect and inhibitory mechanism of oxytetracycline on aerobic denitrification bacteria Acinetobacter sp. AD1 based on cell state, electron behavior and intracellular environment

Article

Author: He, Ting ; Bao, Jianguo ; Cheng, Benai ; Zhou, Yu ; Mao, Qidi ; Zhang, Yi ; Du, Jiangkun

The wide application and low utilization rate of oxytetracycline (OTC) make it often detected in wastewater, which may cause harmful effects on microbial denitrification. Aerobic denitrification (AD) as a new microbial denitrification technology has obvious advantages. However, systematic studies on the effects of OTC on it are lacking. In this study, the effect of OTC on AD was comprehensively explored from multiple perspectives, the main results are as follows. From the perspective of bacterial performance, OTC inhibited AD bacteria growth, denitrification efficiency, and caused serious damage to cell morphological structure, results of CCK-8 confirmed that bacterial activity was significantly affected. From the perspective of electron behavior, OTC decreased electron-producing capacity of carbon metabolism, reduced activity of the electron transport system, inhibited the electron consumption of NAR and NIR to varying degrees, thus increased the risk of nitrite accumulation. From the perspective of intracellular environment, OTC broke redox balance and antioxidant mechanism, related carbon and nitrogen cycle functional genes were down-regulated, affected amino acid, organic acid and nucleotide metabolic processes. The above results provide important information for evaluating the potential risks of antibiotics on the application of AD, and provide key background and theoretical support for stabilizing the technology.

Analysis

Perform a panoramic analysis of this field.

view full example data

AI Agents Built for Biopharma Breakthroughs

Accelerate discovery. Empower decisions. Transform outcomes.

Get started for free today!

Accelerate Strategic R&D decision making with Synapse, PatSnap’s AI-powered Connected Innovation Intelligence Platform Built for Life Sciences Professionals.

Start your data trial now!

Synapse data is also accessible to external entities via APIs or data packages. Empower better decisions with the latest in pharmaceutical intelligence.

Bio

Bio Sequences Search & Analysis

Chemical

Chemical Structures Search & Analysis

CPSF4

Basic Info

Related

Analysis