Where to Buy Authenticated HEK293T Cells with Low Passage Number

When it comes to purchasing authenticated HEK293T cells with a low passage number, researchers need to ensure that they are sourcing their cells from reputable suppliers to guarantee quality and reliability. HEK293T cells, derived from the human embryonic kidney cells, are widely used in scientific research, particularly for gene expression studies and production of recombinant proteins. The integrity and authenticity of these cells are crucial for the validity of experimental outcomes.

One of the most trusted sources for obtaining authenticated HEK293T cells is the American Type Culture Collection (ATCC). ATCC is renowned for providing high-quality, authenticated cell lines to the scientific community. Each batch of HEK293T cells from ATCC undergoes rigorous testing for contamination, identity verification using DNA profiling, and characterization to ensure they meet the standard requirements. Importantly, ATCC provides detailed passage history, allowing researchers to choose cells with low passage numbers, which is essential for minimizing genetic drift and maintaining cell line characteristics.

Another reputable supplier is Sigma-Aldrich, part of Merck KGaA, Darmstadt, Germany. Sigma-Aldrich offers authenticated HEK293T cells that are thoroughly tested for mycoplasma contamination and characterized for surface markers and gene expression profiles. They provide comprehensive documentation to support the authenticity and quality of their cell lines. With a network of global distribution, Sigma-Aldrich ensures that researchers worldwide have access to reliable HEK293T cells with low passage numbers.

Thermo Fisher Scientific, a leader in the scientific supply industry, also offers authenticated HEK293T cells. Their cells are subjected to stringent quality control measures, including STR profiling, to confirm the identity of the cell line. Thermo Fisher provides detailed information regarding the passage number of their HEK293T cells, ensuring researchers can select the most suitable cells for their specific applications. Furthermore, their customer support and technical expertise are invaluable resources for researchers needing guidance on best practices for culturing and handling cells.

In addition to these prominent suppliers, regional cell banks can be an excellent source for acquiring authenticated HEK293T cells with low passage numbers. For instance, the European Collection of Authenticated Cell Cultures (ECACC) and the Japanese Collection of Research Bioresources (JCRB) offer a range of cell lines, including HEK293T cells, which are authenticated and accompanied by comprehensive documentation. These regional cell banks adhere to international standards for cell line authentication, ensuring that researchers are equipped with reliable resources for their scientific endeavors.

When selecting a supplier, it is crucial for researchers to consider factors such as the supplier's reputation, the cell line's passage history, and the availability of detailed authentication documentation. Opting for cells with a low passage number is vital for preserving the genetic and phenotypic integrity of the cell line, which in turn, enhances the reproducibility and reliability of experimental results.

In conclusion, purchasing authenticated HEK293T cells with low passage numbers is an essential step for researchers aiming to achieve robust and reproducible results. By sourcing cells from reputable suppliers such as ATCC, Sigma-Aldrich, Thermo Fisher Scientific, or regional cell banks like ECACC and JCRB, scientists can be confident in the quality and authenticity of their cell lines. This informed choice significantly contributes to the success of their research and the advancement of scientific knowledge.