Delving into the Latest Updates on Ulinastatin-Fc(Takeda Pharmaceutical Co., Ltd.) with Synapse

Fluorescent cholangiography (FC) with indocyanine green (ICG) is an alternative to intraoperative cholangiography (IOC) for visualizing the biliary tract during surgery. This pilot study assessed the feasibility of ICG-FC using inguinal nodal injection in patients undergoing upper gastrointestinal cancer surgery. Under sonographic guidance, ICG was injected into inguinal nodes bilaterally (each side 2.5 mg, total 5 mg) after induction of anesthesia. Laparoscopic cholecystectomy (CHE) and laparoscopic hepatoduodenal ligament clearance (HDL) were performed in 24 cases. ICG-FC was utilized for real-time visualization of extrahepatic biliary anatomy. In CHE cases, ICG-FC provided 100% visualization of the gall bladder (GB), common bile duct (CBD), common hepatic duct (CHD), cystic duct (CD), and CD-CBD junction. For HDL, the visualization rates were 100% for the GB, CHD, and CBD, but only 50% for the undissected CD. Fluorescence appeared in the biliary tree soon after injection and remained throughout the duration of surgery with minimal interference from background hepatic fluorescence. No adverse reactions to ICG were observed. ICG-FC via the inguinal nodal route is safe and feasible, enabling real-time dissection of the calot's triangle and hepatoduodenal ligament. It offers reliable visualization of key biliary structures, showing promise as an alternative to IOC in upper gastrointestinal cancer surgeries.

01 Mar 2024·The Journal of Allergy and Clinical Immunology: In Practice

Home-Based Double-Blind, Placebo-Controlled Challenges for Diagnosis of Delayed Gluten/Milk Hypersensitivity in Children

Article

Author: Landaas, Vibeke Østberg ; Størdal, Ketil ; Carlsen, Karin C Lødrup ; Håland, Geir ; Skjerven, Håvard Ove

BACKGROUNDDelayed hypersensitivity to gluten and milk protein is frequently reported but may be difficult to diagnose.OBJECTIVEWe aimed to explore if a method of home-based double-blind placebo-controlled food challenges (H-FC) can identify and reduce unnecessary elimination diets in children.METHODSWe included 73 of 92 children aged 1 to 17 years referred to a tertiary allergy clinic from 2011 to 2021 due to self-reported, delayed symptoms to gluten or milk. The children were randomized to H-FC, receiving gluten/milk protein or placebo for 5 to 7 days in a double-blind crossover manner, separated by 3 washout weeks. Patients/parents recorded symptoms using standardized forms. Two crossover periods were used from 2011 to 2016 and 3 periods from 2017 to 2021. A positive challenge required significantly more symptoms during the active period versus the placebo period. After the challenge, reintroduction of milk/gluten was assessed by a follow-up interview. The primary outcome was the proportion of children with a positive challenge.RESULTSThe children, with a mean age of 11 years, had followed a strict gluten-free or milk-protein-free diet for a median duration of 24 months (range: 3-180 months). A positive challenge was observed in 18 of 73 children (25%), more often using 2 (35%) compared with 3 challenge periods (12%). At follow-up, 44 of 55 (80%) children with a negative challenge had successfully reintroduced milk/gluten.CONCLUSIONSH-FC may be an effective method in avoiding unnecessary elimination diets in children. Only 25% of the challenges were positive, and 80% of the children with negative challenges succeeded in reintroducing the food. Three challenge periods may be necessary to reduce false-positive outcomes.

15 Nov 2023·Biosensors & bioelectronics

Selenium and nitrogen co-doped carbon dots with highly efficient electrochemiluminescence for ultrasensitive detection of microRNA.

Article

Author: Chai, Yaqin ; Yuan, Ruo ; Liu, Linlei ; Chen, Jie ; Zhang, Yue ; Liu, Jie ; Guo, YuZhuo

In this work, selenium and nitrogen co-doped carbon dots (SeN-CDs) possessing highly efficient electrochemiluminescence (ECL) and excellent biocompatibility were synthesized as a new emitter with S₂O₈^2- as a coreactant for constructing a biosensor to detect microRNA-221 (miRNA-221) sensitively. Notably, the SeN-CDs exhibited superior ECL performance compared with the N-doped CDs, in which selenium with excellent redox activity served as a coreaction accelerator for facilitating the electroreduction of S₂O₈^2- to significantly improve ECL efficiency. Furthermore, target-induced T7 exonuclease (T7 Exo)-assisted double cycle amplification strategy could convert traces of target miRNA-221 into large amounts of output DNA to capture three-dimensional (3D) nanostructures (DTN-Au NPs-DOX-Fc) loaded with large amounts of ECL signal quencher. The constructed biosensor could realize ultrasensitive detection of miRNA-221 and has a low detection limit reaching 2.3 aM, with a successful application to detect miRNA-221 in lysate of Hela and MHCC97-L cancer cell. This work explored a novel method to strengthen the ECL performance of CDs to construct an ECL biosensing platform with sensitive detecting of biomarkers and disease diagnosis.

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