Synergistic effect of electrolyzed water generated by sodium chloride combined with dimethyl dicarbonate for inactivation of Listeria monocytogenes on lettuce

Article

Author: Tian, Shiyi ; Lu, Haixia ; Zhu, Junli ; Shi, Honghui ; Li, Chunliu

AbstractBACKGROUNDElectrolyzed water (EW) is recognized as an effective way to control and reduce pathogens in vegetables. However, the disinfection efficacy of EW alone is limited. In this work, the bactericidal activity and biofilm removal capability of EW, generated by adding NaCl to a portable EW generator, were investigated with special reference to Listeria monocytogenes. Furthermore, the impact of EW in combination with dimethyl dicarbonate (DMDC) in reducing the microbial load and improving the overall quality of lettuce during refrigerated storage was evaluated.RESULTSEW with 0.3% NaCl (SEW) had the highest bactericidal activity against L. monocytogenes. The pathogen treated with SEW exhibited lower superoxide dismutase activity and more leakage of proteins and nucleic acids than in the case of EW. Furthermore, the use of SEW resulted in changes in the cell permeability and morphology of L. monocytogenes. A decrease in adhesion and collapse of the biofilm architecture were also observed, indicating that SEW was more effective for inactivating L. monocytogenes cells compared to EW. For untreated lettuce, the populations of the total plate count and inoculated L. monocytogenes decreased by 2.47 and 2.35 log CFU g−1, respectively, after the combined SEW/DMDC treatment for 3 min. The use of SEW alone or combined with DMDC did not negatively impact the lettuce color values, titratable acid, ascorbic acid and soluble solids compared to the control group.CONCLUSIONSEW in combination with DMDC can be used as a novel and potentially effective disinfection strategy for ensuring the safety of vegetable consumption. © 2023 Society of Chemical Industry.

01 Jun 2022·Environmental Microbiology

Recent insights into oceanic dimethylsulfoniopropionate biosynthesis and catabolism

Review

Author: Shaw, Deepak Kumar ; Ramalingam, Prabavathy Vaiyapuri ; Sekar, Jegan

SummaryDimethylsulfoniopropionate (DMSP), a globally important organosulfur compound is produced in prodigious amounts (2.0 Pg sulfur) annually in the marine environment by phytoplankton, macroalgae, heterotrophic bacteria, some corals and certain higher plants. It is an important marine osmolyte and a major precursor molecule for the production of climate‐active volatile gas dimethyl sulfide (DMS). DMSP synthesis take place via three pathways: a transamination ‘pathway‐’ in some marine bacteria and algae, a Met‐methylation ‘pathway‐’ in angiosperms and bacteria and a decarboxylation ‘pathway‐’ in the dinoflagellate, Crypthecodinium. The enzymes DSYB and TpMMT are involved in the DMSP biosynthesis in eukaryotes while marine heterotrophic bacteria engage key enzymes such as DsyB and MmtN. Several marine bacterial communities import DMSP and degrade it via cleavage or demethylation pathways or oxidation pathway, thereby generating DMS, methanethiol, and dimethylsulfoxonium propionate, respectively. DMSP is cleaved through diverse DMSP lyase enzymes in bacteria and via Alma1 enzyme in phytoplankton. The demethylation pathway involves four different enzymes, namely DmdA, DmdB, DmdC and DmdD/AcuH. However, enzymes involved in the oxidation pathway have not been yet identified. We reviewed the recent advances on the synthesis and catabolism of DMSP and enzymes that are involved in these processes.

21 Apr 2022·Infection and Immunity

N , N -Dimethyldithiocarbamate Elicits Pneumococcal Hypersensitivity to Copper and Macrophage-Mediated Clearance

Article

Author: Pok, Chansorena ; Cutcliffe, Madeline P. ; Wu, Hsin-Jung Joyce ; Neubert, Miranda J. ; Watson, Alison ; Sanchez-Rosario, Yamil ; Ochoa, Klariza ; Menghani, Sanjay V. ; Johnson, Michael D. L.

100 Deals associated with DMDC

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Indication	Highest Phase	Country/Location	Organization	Date
Colorectal Cancer	Phase 3	-	F. Hoffmann-La Roche Ltd.	-
Colorectal Cancer	Phase 3	-	Yamasa Corp.	-
Colorectal Cancer	Phase 3	-	Mitsubishi Tanabe Pharma Corp.	-
Lung Cancer	Phase 3	US	-	-
Non-Small Cell Lung Cancer	Phase 3	-	Yamasa Corp.	-
Non-Small Cell Lung Cancer	Phase 3	-	Mitsubishi Tanabe Pharma Corp.	-
Non-Small Cell Lung Cancer	Phase 3	-	F. Hoffmann-La Roche Ltd.	-

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