Author: Carmen Gómez-Guillén, M ; Sila, Assaad ; Abdallah, Rihab Ben ; Boudawara, Tahia ; Krichen, Fatma ; Ghlissi, Zohra ; Bougatef, Ali ; Sahnoun, Zouheir ; Kallel, Rim ; Martinez-Alvarez, Oscar

The present study aims to evaluate for the first time the wound healing and in vivo anti-inflammatory effects of glycosaminoglycans from skins of smooth hound (SHSG) and grey triggerfish (GTSG). Thermal analysis of GTSG and SHSG was evaluated using differential scanning calorimetry (DSC). The rheologie properties and water absorption capacity of two gels prepared from SHSG and GTSG were also studied. The application of GTSG and SHSG based gels on dermal full-thickness excision wounds in a mouse model, enhanced significantly wound healing activity and a total closure was achieved after eleven days of wound induction for SHSG. Further, histological examination of biopsies showed advanced tissue regeneration, characterized by the presence of well-organized stratum of both derma and epidermis. The anti-inflammatory evaluation of GTSG and SHSG in mice showed a significant inhibition of edema paw, after 5 h of carrageenan injection. The edema inhibition was 91.6% and 90% for SHSG and GTSG, respectively at the dose of 50 mg/kg. Furthermore, the histological evaluation and the superoxide dismutase, catalase and malondialdehyde level in muscle tissue were investigated. In summary, this work demonstrates that both GTSG and SHSG could be promising drugs with good wound healing and anti-inflammatory effects in animal model.

01 Feb 2017·International Journal of Biological MacromoleculesQ1 · CHEMISTRY

Purification, structural characterization and antiproliferative properties of chondroitin sulfate/dermatan sulfate from tunisian fish skins

Q1 · CHEMISTRY

Article

Author: Ali Bougatef ; Nicola Volpi ; Fatma Krichen ; Semia Ellouz-Chaabouni ; Assaâd Sila ; Veronica Mantovani ; Francesca Maccari ; Fabio Galeotti

Chondroitin sulfate/dermatan sulfate GAGs were extracted and purified from the skins of grey triggerfish (GTSG) and smooth hound (SHSG). The disaccharide composition produced by chondroitinase ABC treatment showed the presence of nonsulfated disaccharide, monosulfated disaccharides ΔDi6S and ΔDi4S, and disulfated disaccharides in different percentages. In particular, the nonsulfated disaccharide ΔDi0S of GTSG and SHSG were 3.5% and 5.5%, respectively, while monosulfated disaccharides ΔDi6S and ΔDi4S were evaluated to be 18.2%, 59% and 14.6%, 47.0%, respectively. Capillary elecrophoresis analysis of GTSG and SHSG contained 99.2% and 95.4% of chondroitin sulfate/dermatan sulfate, respectively. PAGE analysis showed a GTSG and SHSG having molecular masses with average values of 41.72KDa and 23.8KDa, respectively. HCT116 cell proliferation was inhibited (p<0.05) by 70.6% and 72.65% at 200μg/mL of GTSG and SHSG respectively. Both GTSG and SHSG demonstrated promising antiproliferative potential, which may be used as a novel, effective agent.

01 Jan 2017·Experimental and Toxicologic Pathology

In vitro and in vivo anti-coagulant activity and toxicological studies of marine sulfated glycosaminoglycans

Article

Author: Sayari, Nadhem ; Krichen, Fatma ; Amor, Ikram Ben ; Boudawara, Tahia ; Ghlissi, Zohra ; Bougatef, Ali ; Gargouri, Jalel ; Kallel, Rim ; Sahnoun, Zouheir

The present study aimed to characterize and evaluate the in vitro and in vivo anticoagulant activity of sulfated glycosaminoglycans from the skins of smooth hound (SHSG) and grey triggerfish (GTSG). The analysis of SHSG and GTSG with acetate cellulose electrophoresis in Zn-acetate revealed the presence of hyaluronic acid (HA), chondroitin sulfate (CS) and dermatan sulfate (DS). Both glycosaminoglycans were evaluated for their in vitro anticoagulant activities using activated partial thromboplastin time (aPTT), thrombin time (TT) and prothrombine time (PT) tests. SHSG and GTSG and calciparin were tested as in vivo anticoagulants by subcutaneous (s.c) injection to adult female Wistar rats in a concentration of 75mg/kg of body weight. The administration of SHSG, GTSG and calciparin to rats induced a significant decrease of platelet rates compared to the control. The aPTT assay of SHSG and GTSG was prolonged 1.3 and 1.23-fold respectively compared with the control. Toxicity studies were performed to investigate whether or not SHSG and GTSG can cause pathological changes in the liver, proteins and DNA. The concentration and catalytic activity of liver oxidative stress markers and enzymes, respectively, as well as the observed hepatic morphological changes indicated that calciparin induced hepatic toxicity and oxidative damage in the liver. The higher activity and lower toxicity of SHSG and GTSG recommended these compounds as a better drug candidate than calciparin.

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Indication	Highest Phase	Country/Location	Organization	Date
Stargardt Disease	Preclinical	Japan	Astellas Pharma, Inc.	09 Mar 2022

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