Delving into the Latest Updates on pPAL-Sfs + pPAL-N with Synapse

Overview

Basic Info

Drug Type

DNA vaccine, Prophylactic vaccine

Synonyms-

Target

SARS-CoV-2 N protein x SARS-CoV-2 S protein

Mechanism

SARS-CoV-2 N protein inhibitors(SARS-CoV-2 N protein inhibitors), SARS-CoV-2 S protein inhibitors(Coronavirus spike glycoprotein inhibitors)

Therapeutic Areas

Infectious Diseases

Active Indication

Coronavirus Infections

Inactive Indication-

Originator Organization

Consejo Superior de Investigaciones Científicas

Active Organization

Consejo Superior de Investigaciones Científicas

Inactive Organization-

Drug Highest PhasePreclinical

First Approval Date-

Regulation-

Author: Alonso, Ana ; Sevilla, Noemí ; Alcolea, Pedro J ; Louloudes-Lázaro, Andrés ; Nogales-Altozano, Pablo ; Redondo, Natalia ; Sánchez-Cordón, Pedro J ; Rojas, José M ; Carlón, Ana B ; Martín, Verónica ; Rodríguez-Martín, Daniel ; Loayza, Francisco J ; Manzano, Miguel ; Palomero, Jesús ; Larraga, Vicente ; Larraga, Jaime ; Ruiz-García, Silvia ; Lozano, Daniel ; Montoya, María ; Vallet-Regí, María

SARS-CoV-2 vaccines currently in use have contributed to controlling the COVID-19 pandemic. Notwithstanding, the high mutation rate, fundamentally in the spike glycoprotein (S), is causing the emergence of new variants. Solely utilizing this antigen is a drawback that may reduce the efficacy of these vaccines. Herein we present a DNA vaccine candidate that contains the genes encoding the S and the nucleocapsid (N) proteins implemented into the non-replicative mammalian expression plasmid vector, pPAL. This plasmid lacks antibiotic resistance genes and contains an alternative selectable marker for production. The S gene sequence was modified to avoid furin cleavage (Sfs). Potent humoral and cellular immune responses were observed in C57BL/6J mice vaccinated with pPAL-Sfs + pPAL-N following a prime/boost regimen by the intramuscular route applying in vivo electroporation. The immunogen fully protected K18-hACE2 mice against a lethal dose (105 PFU) of SARS-CoV-2. Viral replication was completely controlled in the lungs, brain, and heart of vaccinated mice. Therefore, pPAL-Sfs + pPAL-N is a promising DNA vaccine candidate for protection from COVID-19.

100 Deals associated with pPAL-Sfs + pPAL-N

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