To increase resistance to important grapevine pathogens, Chardonnay was transformed with a construct containing two antimicrobial peptides (AMPs), Magainin-2 and PGL.Five lines expressing Magainin-2 transcripts showed increased resistance to two strains of Agrobacterium vitis, TM4 and CG450.These results led to a comprehensive study of the effects of nine AMPs on in vitro growth of four pathogens: two bacterial (A. tumefaciens and A. vitis strains CG450 and TM4) and two fungal (Botrytis cinerea and Erysiphe necator).Cecropin B, MSI-99, and Shiva 1 were most effective against the two A. vitis strains, but ESF-12 had no effect.The growth of TM4 was always more sensitive to AMPs than was CG450, a pattern commensurate with the observations of crown gall resistance among transformed vines expressing Magainin-2.Botrytis cinerea required more than 10 μM α-Purothionin, PGL, or ESF-39 for effective growth inhibition.None of the AMPs studied could reproducibly control E. necator spore germination or lesion formation.MSI-99 and Cecropin B were most promising for future increased resistance to crown gall disease in transgenic grapevines.

01 Jan 2004·Plant Cell ReportsQ2 · BIOLOGY

Expression of a magainin-type antimicrobial peptide gene (MSI-99) in tomato enhances resistance to bacterial speck disease

Q2 · BIOLOGY

Article

Author: A. R. Alan ; E. D. Earle ; A. Blowers

MSI-99 is a synthetic analog of magainin II (MII), a small cationic peptide highly inhibitory to a wide spectrum of microbial organisms. Tomato plants were transformed to express a gene encoding the MSI-99 peptide and tested for possible enhancement of resistance to important pathogens of this crop. Thirty-six tomato transformants carrying an MSI-99 expression vector designed to target the peptide into extracellular spaces were obtained by Agrobacterium tumefaciens-mediated transformation. Expression of MSI-99 caused no obvious cytotoxic effects in these plants. In the tests with Pseudomonas syringae pv. tomato (bacterial speck pathogen) at 10(5 )CFU/ml, several MSI-99-expressing lines developed significantly fewer disease symptoms than controls. However, MSI-99-expressing lines were not significantly different from controls in their responses to the fungal pathogen Alternaria solani (early blight) and the oomycete pathogen Phytophthora infestans (late blight). These findings are in accordance with our previous in vitro inhibition tests, which showed that the MSI-99 peptide is more inhibitory against bacteria than against fungi and oomycetes. Additional in vitro inhibition assays showed that MSI-99 loses its antimicrobial activity in the total or extracellular fluids from leaflets of non-transformed tomato plants; however, P. syringae pv. tomato could not multiply in the extracellular fluid from an MSI-99-expressing line. Our results suggest that expression strategies providing continuous high expression of MSI-99 will be necessary to achieve significant enhancement of plant disease resistance.

01 Feb 2003·PlantaQ2 · BIOLOGY

MSI-99, a magainin analogue, imparts enhanced disease resistance in transgenic tobacco and banana

Q2 · BIOLOGY

Article

Author: V. A. Bapat ; P. K. Mukherjee ; T. R. Ganapathi ; A. Chakrabarti

Magainin is one of the earliest reported antimicrobial peptides isolated from skin secretions of the African clawed frog Xenopus laevis. A synthetic substitution analogue of magainin, MSI-99, is employed in this study to impart disease resistance in transgenic tobacco ( Nicotiana tabacumL.) and banana [( Musaspp. cv. Rasthali (AAB)]. This peptide inhibited the growth and spore germination of Fusarium oxysporumf.sp. cubenseat 16 micro g/ml. MSI-99 has been subcloned into plant expression vectors pMSI164 and pMSI168, targeting the peptide into the cytoplasm and extracellular spaces, respectively. Tobacco plants transformed with pMSI168 showed enhanced resistance against Sclerotinia sclerotiorum, Alternaria alternataand Botrytis cinerea. Transgenic banana pants were obtained for both pMSI164 and pMSI168 transformations and showed resistance to F. oxysporumf.sp. cubenseand Mycosphaerella musicola. The transgenic nature of the transformants and expression of this peptide was confirmed through polymerase chain reaction (PCR) and reverse transcription (RT)-PCR. The results suggest that MSI-99 can be useful in imparting enhanced disease resistance in transgenic plants.

100 Deals associated with MSI-99

R&D Status

10 top R&D records.

to view more data

Indication	Highest Phase	Country/Location	Organization	Date
Carcinoma	Preclinical	United States	Genaera Corp.	-
Carcinoma	Preclinical	Global	Sandoz AG	-
Neoplasms	Preclinical	United States	-	-

Clinical Result

Indication

Phase

Evaluation

View All Results

Translational Medicine

Boost your research with our translational medicine data.

view full example data

Deal

Boost your decision using our deal data.

view full example data

Core Patent

Boost your research with our Core Patent data.

view full example data

Clinical Trial

Identify the latest clinical trials across global registries.

view full example data

Approval

Accelerate your research with the latest regulatory approval information.

view full example data

Regulation

Understand key drug designations in just a few clicks with Synapse.

view full example data

AI Agents Built for Biopharma Breakthroughs

Accelerate discovery. Empower decisions. Transform outcomes.

Get started for free today!

Accelerate Strategic R&D decision making with Synapse, PatSnap’s AI-powered Connected Innovation Intelligence Platform Built for Life Sciences Professionals.

Start your data trial now!

Synapse data is also accessible to external entities via APIs or data packages. Empower better decisions with the latest in pharmaceutical intelligence.

Bio

Bio Sequences Search & Analysis

Chemical

Chemical Structures Search & Analysis


No Data

MSI-99

Overview

Basic Info

Structure/Sequence

Related

R&D Status

Clinical Result

Translational Medicine

Deal

Core Patent

Clinical Trial

Approval

Regulation