CJM 126

Three benzo-nitrogen-containing heterocyclic probes with continuously tuned characteristics were designed to detect γ-Glutamyltranspeptidase (GGT) activity.The specific recognition site is the γ-glutamine bond, which is a specific chem. bond, and the activity of GGT leads to the hydrolysis of this bond.Probe 1 was synthesized by designing ′ d-π-A ′ structure and using benzimidazole acetonitrile as a fluorescent group.The UV absorption change was obvious and the fluorescence intensity was extremely weak.The detection limit was as low as 0.0608 U/mL.Under natural light, the probe solution gradually changed from colorless to slightly yellow with the increase of GGT activity.The benzo-pyrrole acetonitrile with weak charge transfer was used as the fluorescent group to synthesize probe 2, and the fluorescence intensity quenching was obvious, its detection limit was 0.244 U/mL.By designing ′ d-π-A ′ structure and benzothiazole with strong charge transfer ability was used as a fluorescent group to design and synthesize probe 3, and the fluorescence enhancement was more obvious.The solution gradually changed from colorless to blue under 365 nm UV irradiationThe enzymic hydrolysis of probe 2, probe 3 take 25 mins to complete.In addition, the B value of probe 1, probe 3 solution and the G value of probe 2 solution were linearly connected with GGT activity, resp.At the same time, it was found that the GGT activity of probe 2 and probe 3 had a linear correlation with E, while probe 1 did not.Therefore, by optimizing the fluorescent group, the performance of the probe and the detection effect are improved.These three probes can be used as a practical tool for identifying GGT activity.