Mafoprazine mesylate

New automatic pretreatment equipment (FASVED; Food Automatic Analytical Systems for Veterinary Drugs) was developed. FASVED consists of ten main units: reagent dispenser, homogenizer, transfer hand, lid opening/closing device, centrifugal separator, pipette, shaker, column purification device, centrifugal evaporator and cooling device, and it is capable of freely combining operations by these units. A validation study was performed on two methods for determination of 178 veterinary drugs in livestock products, swine muscle, egg and shrimp, according to the method validation guideline of the Ministry of Health, Labour and Welfare of Japan. The numbers of analytes that satisfied the criteria of the guideline were 148 in swine muscle, 160 in egg and 151 in shrimp.

A simultaneous determination of veterinary drugs in livestock food and seafood using liquid chromatography with tandem mass spectrometry (LC/MS/MS) was developed. Veterinary drugs were extracted with 95% acetonitrile. The solution was passed through a Florisil column, and the solvent was replaced with phosphate buffer. The extract was charged on a Sep-Pak Plus C(18) mini-column and divided into 40% methanol eluate fraction and 70% acetonitrile eluate fraction. Test solutions were analyzed by LC/MS/MS with gradient elution. By using this method, 37 kinds of veterinary drugs were obtained with over 60% recovery, and quantitation was possible in cattle muscle, egg and fish. This method was inapplicable to 28 kinds of veterinary drugs. Although quantitation was not achieved, 42 other kinds of veterinary drugs can be screened. Since the limit of quantitation for this method is less than the provisional limit in general, it is useful as a screening method in residual analysis of veterinary drugs.