Delving into the Latest Updates on AFG-2 with Synapse

Overview

Basic Info

Drug Type

Small molecule drug

Synonyms

anti-VEGF Nanofitins (Affilogic), AFG-2

Target

VEGFR

Action

antagonists

Mechanism

VEGFR antagonists(Vascular endothelial growth factor receptor antagonists)

Therapeutic Areas

Eye Diseases

Active Indication-

Inactive Indication

Eye Diseases

Originator Organization

Affilogic Laboratories

Active Organization-

Inactive Organization

Affilogic Laboratories

License Organization-

Drug Highest PhasePendingDiscovery

First Approval Date-

Regulation-

Aflatoxins (AFs) such as AFB₁, AFB₂, AFG₁, and AFG₂ are some of the main sources of exogenous contamination in traditional Chinese medicines (TCMs). Contamination by these four AFs can occur in the process of planting, processing, production, storage, and circulation, and will affect the safety of TCM applications. In this work, we synthesized a macroporous covalent organic framework (COF) as the adsorbent for the online solid-phase extraction (SPE) of the four AFs. Monodisperse polystyrene microspheres were used as the sacrificial template for the creation of macropores. The physical properties of macroporous COF were characterized. The online SPE processes were optimized. The online SPE method coupled with a high-performance liquid chromatograph equipped with a UV detector was used to determine the amount of the four AFs in TCM samples, including lotus seed, Sterculia lychnophora, coix seed, Ziziphi spinosae semen, and malt. Using the optimized method, a linear calibration curve was obtained in the range of 0.05-100 ng/g. The limits of detection were 0.015-0.03 ng/g. The recoveries of the four AFs in TCM samples ranged from 80.0 % to 101 %. This study presents a feasible and effective approach for the detection of AFs at trace level.

01 Apr 2025·FOOD CHEMISTRY

A stable and reusable aluminum-based metal-organic framework for the effective extraction of four aflatoxins from vegetable oils

Article

Author: Jiang, Xianhong ; Yang, Zhihui ; Cheng, Jie ; Wang, Peilong

The high specific surface area of metal-organic framework (MOF) materials endows them with efficient adsorption capabilities, thereby facilitating sample purification. In this study, a novel aluminum-based MOF (Al-MOF) was synthesized and employed as a solid-phase extraction (SPE) adsorbent for the purification of aflatoxins B₁ (AFB₁), AFB₂, AFG₁, and AFG₂ in vegetable oils. It was revealed that Al-MOF adsorbs aflatoxins through hydrogen bonding and π-π interactions. Under optimal SPE conditions, liquid chromatography-tandem mass spectrometry analysis yielded limits of detection ranging from 0.06 to 0.25 μg/kg and limits of quantification from 0.21 to 0.84 μg/kg for the four aflatoxins. Recovery rates at concentrations of 5, 10, and 20 μg/kg ranged from 74 % to 110 %, with coefficients of variation below 11 %. This method achieves efficient and cost-effective purification of aflatoxins in vegetable oils. Compared to national standard methods, this approach offers advantages such as lower material costs, ease of storage, and reusability.

01 Mar 2025·JOURNAL OF AOAC INTERNATIONAL

Validation of the 11+Myco MS-PREP® Method for Determination of Aflatoxins, Fumonisins, Deoxynivalenol, Ochratoxin A, Zearalenone, HT-2, and T-2 Toxins in Cereals, Baby Food, Spices, and Animal Feed by Immunoaffinity Column with LC–MS/MS: AOAC Performance Tested MethodSM 112401

Article

Author: Allan, Andrew B ; Stratton, Joanna ; Leeman, Dave ; MacDonald, Susan J ; Tramaseur, Adam ; Cameron, Helen ; Donelly, Carol

Abstract:

Background:

The 11+Myco MS-PREP® immunoaffinity column (IAC) contains a gel suspension of monoclonal antibodies specific to the toxins of interest. Following sample extraction, the IAC is used for cleanup and concentration of mycotoxins prior to analysis by LC with tandem mass spectrometry (LC–MS/MS).

Objective:

This study evaluated the IAC with LC–MS/MS method for Performance Tested MethodSM certification for simultaneous determination and confirmation of aflatoxins (AF) B1, B2, G1, G2, and M1; deoxynivalenol (DON), fumonisins B1, B2, and B3; ochratoxin A (OTA); T-2; HT-2; and zearalenone (ZON) from corn, wheat, cereal-based baby food (with and without dairy ingredients), paprika, chili powder, and animal feed.

Methods:

A single extraction method using acetonitrile-water (1 + 1, by volume) was used for all matrixes. The method developer validated all matrixes and an independent laboratory verified method performance on corn and animal feed. Data were analyzed for recovery, repeatability precision, LOD, LOQ, confirmation of identity, and method selectivity.

Results:

Recovery (72–138%) and repeatability (0.46–24%), with the exception of sporadic data points, were within acceptance criteria. LOQ was estimated as AFB1 0.018–0.32 μg/kg, AFB2 0.037–0.28 μg/kg, AFG1 0.019–0.14 μg/kg, AFG2 0.036–0.28 μg/kg, DON 4.0–75 μg/kg, fumonisin B1 4.9–37 μg/kg, fumonisin B2 4.0–32 μg/kg, fumonisin B3 2.0–16 μg/kg, OTA 0.15–4.4 μg/kg, T-2 0.5–7.5 μg/kg, HT-2 0.70–7.5 μg/kg, and ZON 1.3–7.2 μg/kg, depending on matrix. Method performance was verified with reference and QC materials. Selectivity and confirmation of identity were also demonstrated.

Conclusion:

The 11+Myco MS-PREP IAC with LC–MS/MS method demonstrated acceptable performance for simultaneous determination of 12 mycotoxins in seven matrixes.

Highlight:

The data were reviewed by the AOAC Performance Tested MethodsSM Program and approval was granted for certification of the 11+Myco MS-PREP Method as PTM 112401.

100 Deals associated with AFG-2

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