Innovative utilization of silver nanoparticles localized surface plasmon resonance for green and sensitive spectrofluorimetric analysis of sildenafil and xipamide in pure forms and pharmaceutical preparations

Article

Author: El-Brashy, Amina M ; Fathy, Mona E ; Barakat, Neamat T

A green, novel, simple and sensitive spectrofluorimetric approach was investigated and validated for the analysis of two important cardiovascular drugs namely; sildenafil citrate and xipamide using silver nanoparticles as a fluorescence probe (Ag-NPs). Silver nanoparticles were prepared through chemical reduction of silver nitrate using sodium borohydride in distilled water without using non-green organic stabilizer. These nanoparticles were stable, water soluble and had high fluorescence. After addition of the studied drugs, noticeable quenching of Ag-NPs fluorescence occurred. The intensity of Ag-NPs fluorescence was measured at 484 nm (λ_ex 242 nm) before and after complex formation with these studied drugs. The difference between these values (ΔF) were linear with the concentrations in the following ranges (1.0-10.0 μg/mL), (0.5-5.0 μg/mL) for sildenafil and xipamide, respectively. The formed complexes did not need to be separated by solvent extraction before measurement. For proving the complex formation between the two studied drugs and silver nanoparticles, stern volmer method was applied. The suggested method was perfectly validated in compliance with the international conference on harmonization (ICH) Guidelines and the outcomes were acceptable. Furthermore, suggested technique was perfectly applied for the assay of each drug in its pharmaceutical dosage form. Eventually assessment of method greenness was performed using different tools and found that the suggested method was safe and eco-friendly.

13 May 2023·BMC chemistry

Univariate versus multivariate spectrophotometric data analysis of triamterene and xipamide; a quantitative and qualitative greenly profiled comparative study.

Article

Author: Zaazaa, Hala E ; Abd El-Hadi, Heidi R ; Eltanany, Basma M ; Eissa, Maya S

Triamterene (TRI) and xipamide (XIP) mixture is used as a binary medication of antihypertension which is considered as a major cause of premature death worldwide. The purpose of this research is the quantitative and qualitative analysis of this binary mixture by green univariate and multivariate spectrophotometric methods. Univariate methods were zero order absorption spectra method (D⁰) and Fourier self-deconvolution (FSD), as TRI was directly determined by D⁰ at 367.0 nm in the range (2.00-10.00 µg/mL), where XIP show no interference. While XIP was determined by FSD at 261.0 nm in the range (2.00-8.00 µg/mL), where TRI show zero crossing. Multivariate methods were Partial Least Squares, Principal Component Regression, Artificial Neural Networks, and Multivariate Curve Resolution-Alternating Least Squares. A training set of 25 mixtures with different quantities of the tested components was used to construct and evaluate them, 3 latent variables were displayed using an experimental design. A set of 18 synthetic mixtures with concentrations ranging from (3.00-7.00 µg/mL) for TRI and (2.00-6.00 µg/mL) for XIP, were used to construct the calibration models. A collection of seven synthetic mixtures with various quantities was applied to build the validation models. All the proposed approaches quantitative analyses were evaluated using recoveries as a percentage, root mean square error of prediction, and standard error of prediction. Strong multivariate statistical tools were presented by these models, and they were used to analyze the combined dosage form available on the Egyptian market. The proposed techniques were evaluated in accordance with ICH recommendations, where they are capable of overcoming challenges including spectral overlaps and collinearity. When the suggested approaches and the published one were statistically compared, there was no discernible difference between them. The green analytical method index and eco-scale tools were applied for assessment of the established models greenness. The suggested techniques can be used in product testing laboratories for standard pharmaceutical analysis of the substances being studied.

04 Feb 2022·Journal of AOAC INTERNATIONALQ4 · AGRICULTURAL & FORESTRY SCIENCE

Ultra-Performance Liquid Chromatographic and Densitometric Methods for Sensitive Determination of Xipamide and Triamterene in Pure and Pharmaceutical Dosage Forms

Q4 · AGRICULTURAL & FORESTRY SCIENCE

Article

Author: Fares, N V ; Badawey, Amr M ; El Fiky, Haitham A ; Abd El Ghany, Maha F

AbstractBackgroundValidated ultra-performance liquid chromatography (UPLC) and thin-layer chromatography (TLC) densitometric methods were prescribed for determination of antihypertensive components.ObjectiveTo establish and validate rapid and accurate UPLC and TLC densitometric methods for determination of Xipamide and Triamterene in pure and dosage forms.MethodsThe first method, UPLC, depended on using an Agilent Zorbax Eclipse Plus C8 (50 mm × 2.1 mm, 1.8 μm) column, a mobile phase composed of acetonitrile-water (70 + 30, v/v) adjusted by acetic acid to obtain pH 3, 0.2 mL/min flow rate, and UV detection at 231.4 nm. The second method was a TLC densitometric method. Separation was achieved by using toluene-methanol-ethyl chloride-acetic acid (7 + 2 + 1 + 0.2, v/v/v) as the mobile phase, pre coated silica gel plates as the stationary phase, and UV detection at 300.0 nm.ResultsThe obtained results were validated and statistically compared with official and reported methods. The obtained results showed high accuracy and reproducible results with excellent mean recoveries for both drugs.ConclusionThe UPLC method showed shorter retention time for both Xipamide (0.88 min) and Triamterene (0.63 min), a lower detection limit of less than 0.055 µg/mL for both drugs with high selectivity, decreased injection volume (1 µL), and a lower flow rate than any HPLC method. Both proposed methods were sensitive, selective, and effectively applied to pure and dosage forms (Epitens®).HighlightsUnprecedented sensitive, rapid, and reproducible UPLC and TLC methods were developed for selective determination of mixtures of Xipamide and Triamterene, with LOD os less than 0.076 µg/mL for both drugs.

100 Deals associated with Xipamide

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KEGG	Wiki	ATC	Drug Bank
D06341	Xipamide	C03BA10	DB13803

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Indication	Country/Location	Organization	Date
Edema	-	-	-
Heart Failure	-	-	-
Hypertension	-	-	-

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