Delving into the Latest Updates on Obesity exosome with Synapse

Overview

Basic Info

Drug Type

Exosomes

Synonyms-

Target-

Mechanism-

Therapeutic Areas

Endocrinology and Metabolic Disease

Active Indication

Obesity

Inactive Indication-

Originator Organization

BioRestorative Therapies, Inc.

Active Organization

BioRestorative Therapies, Inc.

Inactive Organization-

Drug Highest PhasePreclinical

First Approval Date-

Regulation-

Diabetic wound is one of the most common and costly complication in diabetic patients. Hence, numerous studies have been carried out to discover a suitable approach to enhance the process of wound healing. Biological hydrogels are commonly utilized for wound healing due to their suitable properties among different materials available. Herein we investigated whether human amniotic membrane hydrogel (hAMH) loaded with human placental mesenchymal stem cells (PlaMSCs)-derived exosomes could promote healing in diabetic rats. Sixty diabetic rats were randomly assigned into the control group, hAMH group, exosome group, and hAMH+Exosome group. According to the phases of wound healing, sampling was done on days 7, 14, and 21 for further assessments. Our findings showed a significant increase in wound contraction rate, new epidermal length, fibroblast and blood vessel count, collagen density, and the levels of antioxidative factors (GSH, SOD, and CAT) in the treatment groups compared to the control group, with more pronounced effects observed in the hAMH+Exosome group. Furthermore, the levels of bFGF and VEGF gene expression significantly increased in each treatment group when compared to the control group, with the highest levels observed in the hAMH+Exosome group. This occurred as the hAMH+Exosome group showed a greater decrease in neutrophil count, the expression of TNF-α and IL-1β genes, and the levels of an oxidative factor (MDA) compared to the other groups. In summary, the combination of hAMH and PlaMSCs-derived exosomes was determined to have a more significant effect on healing diabetic wounds.

01 Aug 2024·AMERICAN JOURNAL OF SPORTS MEDICINE

Skeletal Stem Cell–Derived Exosomes Promote Meniscal Tear Healing and Ameliorate Secondary Osteoarthritis

Article

Author: Jiang, Dong ; Du, Jing-Ke ; Chen, Rao ; Dou, Yun ; Du, Ming-Ze ; Zhang, Fang-Xue ; Chien, Meng-Han ; Zhang, Bo ; Ai, Li-Ya ; Zhang, Zhen

Background::

The self-repair ability after meniscal tears is poor, leading to the development of posttraumatic osteoarthritis. Promoting the repair of meniscal injuries remains a great challenge, especially in the avascular region.

Hypothesis::

Local delivery of skeletal stem cell (SSC)–derived exosomes (SSC-Exos) would promote meniscal healing and prevent secondary osteoarthritis progression.

Study Design::

Controlled laboratory study.

Methods::

SSCs were isolated from bone marrow and exosomes were extracted via ultracentrifugation. The cell migration capabilities after incubation with exosomes were validated through in vitro cell culture. Full-thickness longitudinal medial meniscal tears were performed in the avascular region of 40 male Sprague-Dawley rats and 20 male New Zealand White rabbits, which were randomly divided into 2 groups: group treated with phosphate-buffered saline (GCON) and group treated with exosomes (GExosome). The effects of these treatments on meniscal healing and secondary osteoarthritis were evaluated by gross inspection, biomechanical testing, and histological assessment. RNA sequencing of in vitro cell cultures was performed to explore the underlying mechanisms.

Results::

Exosomes were successfully extracted and identified. These exosomes significantly promoted cell migration capabilities in vitro ( P < .01). The GExosome exhibited greater cell proliferation and tissue regeneration with type 2 collagen secretion, and a significantly higher meniscal repair score than that of the GCON at 8 weeks postoperatively ( P < .05). In contrast to the degenerative changes in both the meniscus and articular cartilage of the GCON, meniscal tissue in the GExosome exhibited restoration of normal morphology with a smooth and glossy white surface and better mechanical strength at 8 weeks after meniscal repair. Both degeneration scores and synovitis scores were significantly higher in the GCON than in the GExosome ( P < .05). Compared with the GCON, the expression of key genes related to cell migration, such as the chemokine family, was enhanced by exosome injection, leading to an upregulation of extracellular matrix expression while downregulating the expression of inflammation-related genes such as CD68 and the matrix metalloproteinase family.

Conclusion::

The administration of SSC-Exos effectively promoted meniscal healing in the avascular region and ameliorated secondary osteoarthritis. The effect might be attributed to inflammation modulation, promotion of cell migration, and secretion of extracellular matrix components.

Clinical Relevance::

Injection of SSC-Exos represents a promising therapeutic option for promoting meniscal healing in the avascular region.

01 Nov 2014·The FEBS journalQ3 · BIOLOGY

Unconventional secretion of synuclein‐γ promotes tumor cell invasion

Q3 · BIOLOGY

Article

Author: Shou, Chengchao ; Lian, Shenyi ; Liu, Caiyun ; Meng, Lin ; Zhao, Chuanke ; Qu, Like ; Tian, Zhihua

Structured digital abstract:

• Flotillin-2 physically interacts with SNCG by anti bait coip (1, 2).

100 Deals associated with Obesity exosome

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