A014(Lanzhou University)

Caseinolytic protease P (ClpP) protein is essential for prokaryotic and eukaryotic protein homeostasis, and has emerged as a promising bactericidal target for controlling bacterial infection and evading the emergent of drug-resistance risk. As the unique bactericidal mechanism, ClpP loses its substrate specificity and undergoes uncontrolled protein hydrolysis in the presence of an activator, leading to causing bacterial death. To further expand the chemotype of ClpP activator, a series of ONC212 derivatives were synthesized using a ring expansion strategy. Surprisingly, compound A₁₄ showed optimal antibacterial activity against Xanthomonas oryzae pv. oryzae (Xoo) in vitro (EC₅₀ value = 3.86 μg/mL), which was >6 × more potent than the EC₅₀ value of ONC212 (26.42 μg/mL). Molecular docking, fluorescence titration and degradation experiments of the XooFtsZ protein indicated that compound A₁₄ was a strong degrader of XooClpP towards XooFtsZ. In vivo assays showed that the control activity of compound A₁₄ (200 μg/mL) reached 47.47 %, compared to 41.57 %, 36.72 %, and 30.43 % for ONC212, thiodiazole copper, and bismerthiazol, respectively. Overall, this study led to the identification compound A₁₄, which not only showed improved antibacterial potency, and maintained the binding XooClpP, but also highlighted the ring expansion strategy as a promising approach for bactericide discovery.