Author: Rodrigues, Michele Angela ; de Melo, Mariane Izabella Abreu ; de Goes, Alfredo Miranda ; de Miranda, Marcelo Coutinho ; da Silva Cunha, Pricila ; Faria, Jerusa Araújo Quintão Arantes ; Gomes, Dawidson Assis ; Barbosa, Joana Lobato

In recent years, stem cell therapy has shown promise in regenerative medicine. The lack of standardized protocols for cell isolation and differentiation generates conflicting results in this field. Mesenchymal stem cells derived from adipose tissue (ASC) and fibroblasts (FIB) share very similar cell membrane markers. In this context, the distinction of mesenchymal stem cells from fibroblasts has been crucial for safe clinical application of these cells. In the present study, we developed aptamers capable of specifically recognize ASC using the Cell-SELEX technique. We tested the affinity of ASC aptamers compared to dermal FIB. Quantitative PCR was advantageous for the in vitro validation of four candidate aptamers. The binding capabilities of Apta 2 and Apta 42 could not distinguish both cell types. At the same time, Apta 21 and Apta 99 showed a better binding capacity to ASC with dissociation constants (Kd) of 50.46 ± 2.28 nM and 72.71 ± 10.3 nM, respectively. However, Apta 21 showed a Kd of 86.78 ± 9.14 nM when incubated with FIB. Therefore, only Apta 99 showed specificity to detect ASC by total internal reflection microscopy (TIRF). This aptamer is a promising tool for the in vitro identification of ASC. These results will help understand the differences between these two cell types for more specific and precise cell therapies.

01 Dec 1990·Journal of Investigative DermatologyQ1 · MEDICINE

Melanocytic Differentiation of Human Neuroblastoma: Expression of a Human Melanosome-Associated Antigen

Q1 · MEDICINE

Article

Author: L Martin Jerry ; Ronald C McGarry ; Kazuo Maeda ; Valter Feyles

Five human neuroblastoma cell lines were examined for expression of a human melanosome-associated antigen (HMSA). Only cell line SK-N-SH reacted with a monoclonal antibody, HMSA-2, shown to recognize melanosomal glycoproteins. To further characterize the melanocytic lineages of SK-N-SH, three morphologically distinct clones designated SK-N-SH-N (neuroblast type), SK-N-SH-F (fibroblast type), and SK-N-SH-EP (epithelial type) were established by colony formation cloning. By fluorescence-activated cell sorter analysis and tyrosinase assay, we found that only SK-N-SH-EP and SK-N-SH-F reacted with HMSA-2 and had tyrosinase activity. These results suggest that epithelial-type and fibroblast-type cells appear to possess the melanocytic potential, but not neuroblast-type cells. Furthermore, SK-N-SH-EP was found to spontaneously convert to neuroblast-type or fibroblast-type cells, whereas SK-N-SH-N and SK-N-SH-F clones have remained morphologically stable. Our results suggest that at least one neuroblastoma cell line, SK-N-SH, may be an excellent model for investigating clonal maturation and the melanocytic differentiation of neuroblastoma.

01 Dec 1987·TalantaQ2 · CHEMISTRY

Analysis of the CCRMP Oka-2 rare-earth reference mineral of the britholite—apatite series by electrothermal atomic-absorption and inductively-coupled plasma atomic-emission spectrometry

Q2 · CHEMISTRY

Article

Author: Gupta, J G

The lanthanides and yttrium in the Canadian Certified Reference Materials Project (CCRMP) new rare-earth mineral reference material, Oka-2, were determined by electrothermal atomic-absorption and inductively-coupled plasma atomic-emission spectrometry (ICPAES) after sample decomposition with acids and separation of the rare-earth metals from phosphate and other matrix elements by precipitation as fluorides and oxalates. Thorium, yttrium and the common major and minor elements were determined by ICPAES after sample decomposition by fusion with lithium meta- and tetraborates and dissolution of the melt in a mixture of dilute nitric acid and ethylenediaminetetra-acetic acid solution. For comparison purposes, silicon, phosphorus, calcium, magnesium and iron were also determined by other methods. The results obtained are compared with other CCRMP values and with those obtained previously for a similar rare-earth mineral from the same geographical area. Oka-2 is considered to be a thorian intermediate member of the britholite-apatite series.

100 Deals associated with Apta-2

R&D Status

10 top R&D records.

to view more data

Indication	Highest Phase	Country/Location	Organization	Date
Inflammation	Preclinical	SE	Aptahem AB	14 Dec 2017

Clinical Result

Indication

Phase

Evaluation

View All Results

Translational Medicine

Boost your research with our translational medicine data.

view full example data

Deal

Boost your decision using our deal data.

view full example data

Core Patent

Boost your research with our Core Patent data.

view full example data

Clinical Trial

Identify the latest clinical trials across global registries.

view full example data

Approval

Accelerate your research with the latest regulatory approval information.

view full example data

Regulation

Understand key drug designations in just a few clicks with Synapse.

view full example data

Chat with Hiro

Get started for free today!

Accelerate Strategic R&D decision making with Synapse, PatSnap’s AI-powered Connected Innovation Intelligence Platform Built for Life Sciences Professionals.

Start your data trial now!

Synapse data is also accessible to external entities via APIs or data packages. Empower better decisions with the latest in pharmaceutical intelligence.

Bio

Bio Sequences Search & Analysis

Chemical

Chemical Structures Search & Analysis


No Data

Apta-2

Overview

Basic Info

Structure/Sequence

Related

R&D Status

Clinical Result

Translational Medicine

Deal

Core Patent

Clinical Trial

Approval

Regulation