Promegapoietin

Isolated pearl millet and sorghum proteins were modified using micronization technique via high shear homogenization at 15000 rpm for 10 mins and characterized for techno-functionality, in vitro protein digestibility, electrokinetic potential, particle size, structural and thermal behavior along with the native proteins. The particle size for micronized pearl millet protein (PMP) and sorghum protein (SP), marked reduction from 4.12 μm to 1.60 μm and 3.07 μm to 1.40 μm, respectively. Micronized proteins exhibited improved functional properties, discerned as improved water and fat absorption capacity (4.14, 4.59, and 3.52, 3.74 g/g respectively), surface hydrophobicity (189.78 and 215.89), solubility (65.34 and 67.92 %), surface properties and biological properties (89.26 and 85.65 %) in comparison to native proteins, respectively. Highest improvement in the functionality were observed for micronized SP, even though micronized PMP showed fairly similar increase in functional properties. Also, the micronized PMP and SP exhibited change in the conformation of protein as alteration in interrelated nuances of FTIR spectra, microstructure, and zeta potential in comparison to native counterparts. Furthermore, results for colorimetric response suggested significant structural changes. The slight decrease in the denaturation enthalpies (Δ_H) values of -0.12 to -0.05 J/g and - 9.22 to -9.09 J/g were observed for micronized PMP and SP, respectively.

Diabetic wounds are characterized by multiple anomalies in the four overlapping phases of wound healing. Therefore, targeting multiple factors is considered ideal for more promising therapeutic outcome. We came across two potent molecules, asiaticoside (AST) and neurotensin (NT), which promote wound healing during different phases. However, AST shows poor physicochemical properties and has high molecular weight, poor solubility, and permeability. NT, on the other hand, is rapidly degraded by proteases present at the wound site, limiting its effectiveness. This study focuses on developing asiaticoside-loaded polymeric nanoparticles (AST PNPs) and neurotensin-conjugated porous microparticles (NT-PMPs), followed by their incorporation into a gelatin-based hydrogel. This codelivery system aims to explore their synergism and hence improve therapeutic outcomes. AST PNPs with a size of 168.4 nm (0.09 PDI) were prepared. NT-PMPs were prepared within a size range of 60-200 μm. Application of the AST PNP&NT-PMP-loaded gelatin gel on wounds of diabetic rats resulted in accelerated healing with completely re-epithelialized epidermis, migrating fibroblasts, and regeneration of hair follicles in the treated group. An organized collagen matrix and significant upregulation of NT (∼4-fold) and COL-1 (∼2.5-fold) levels compared to untreated animals were noted. Additionally, relative mRNA expression of key healing markers eNOS increased by 6.97-fold and MMP-9 decreased by 4.83-fold in the AST PNP&NT-PMP hydrogel-treated group compared to the untreated animals. Altogether, AST encapsulated into polymeric nanoparticles and NT conjugated to PMP, both loaded in gelatin gel, depicted significant improvement due to their synergistic behavior in therapeutic response compared to individual molecules and untreated groups.