Probiotics and their cellular constituents are pivotal in modulating lipid metabolism in adipocytes, potentially offering novel approaches to obesity prevention. This study investigates the probiotic characteristics and anti-adipogenic effects of lactic acid bacteria (LAB) strains isolated from kimchi. Twenty LAB strains, identified as Latilactobacillus sakei, Latilactobacillus curvatus, and Leuconostoc mesenteroides, were evaluated for lipase inhibitory activity. Among them, L. sakei CKC1 and L. curvatus CK17 exhibited the strongest inhibition and were selected for further study. Notably, L. curvatus CK17 significantly reduced lipid accumulation in 3T3-L1 adipocytes and downregulated key adipogenic markers, including PPARγ, C/EBPα, aP2, SREBP1c, ACC, and FAS (p < 0.05). Additionally, CK17 survived simulated digestive conditions, adhered effectively to Caco-2 cells, and exhibited antibacterial activity. These findings suggest that L. curvatus CK17 as a promising probiotic candidate for obesity prevention, with potent anti-adipogenic properties.

18 Mar 2016·International Journal of OphthalmologyQ4 · MEDICINE

Effect of cytokeratin 17 on retinal pigment epithelium degeneration and choroidal neovascularization

Q4 · MEDICINE

ArticleOA

Author: Zhuang, Pei ; Xiao, Tao ; Shen, Yi ; Chiou, George Cy

AIMTo study the effects of cytokeratin 17 (CK17) on sodium iodate (NaIO3) induced rat retinal pigment epithelium (RPE) degeneration, laser induced rat choroidal neovascularization (CNV), and oxidative stress of human retinal pigment epithelium cells (ARPE-19) and human umbilical vein endothelial cell (HUVEC).METHODSThirty 8-week-old male Brown Norway rats were randomly divided into 3 groups, 10 rats in control group treated with solvent alone; 10 rats in NaIO3 group treated with solvent and 35 mg/kg NaIO3 injection through hypoglossal vein and 10 rats in CK17+NaIO3 group treated with 1% CK17 eye drop 3 times a day for 1wk before and 4wk after NaIO3 injection. RPE function was measured with c-wave of electroretinogram (ERG). Another 20 rats were randomly divided into 2 groups. Of them 10 rats in CK17 group were anesthetized to receive Nd:YAG laser and given 1% CK17 eye drop before same as above; 10 rats in control were received Nd:YAG and treated with solvent. The development of choroidal neovascularization (CNV) was determined by fundus fluorescein angiography (FFA) performed on 4wk after laser. Methylthiazoly tetrazolium (MTT) assay was used to study effect of CK17 on various oxidants induced injury in ARPE-19 and HUVEC in vitro.RESULTSFour weeks after NaIO3 injection, the c-wave amplitude of ERG was 0.393±0.02 V in the control group, 0.184±0.018 V in NaIO3 group and 0.3±0.01 V in CK17+NaIO3 group. There was a significant reversal of the c-wave by CK17 as compared to NaIO3 group (P<0.01). Four weeks after laser, the size of the CNV lesion was 2.57±0.27 mm(2) in control group and 1.64±0.08 mm(2) in CK17 group. The lesion size significantly diminished in CK17 group (P<0.01). The in vitro results showed CK17 also reversed the various oxidants induced injuries in ARPE-19 at the dose of 100 µg/mL and enhanced the injury in HUVECs at different concentrations.CONCLUSIONCK17 can significantly protect RPE from NaIO3 induced degeneration in vivo and in vitro and also could reverse the various oxidants induced injuries in vitro. It inhibits the development of CNV in rat model, interfered with vascular endothelial cell proliferation in vitro.

01 May 1999·Zhongguo yao li xue bao = Acta pharmacologica Sinica

Systemic anti-inflammation by synthetic interleukin-1 blockers.

Article

Author: Liu, S X ; Chiou, G C

AIMTo study the systemic anti-inflammatory actions of interleukin-1 (IL-1) blockers, OB-101 and OB-186.METHODSPrevention of palm swelling induced by carrageenin injection was used as an animal model of systemic anti-inflammation efficacy.RESULTSBoth OB-101 and OB-186 (10-30 mg.kg-1) were approximately 10-30-fold more potent than aspirin (300 mg.kg-1) to inhibit carrageenin-induced systemic inflammation. The LD50 of OB-101 and OB-186 were at least 20 g.kg-1 i.g., indicating that they were extremely safe agents with a therapeutic index (LD50/ED50) of at least 2000.CONCLUSIONThese IL-1 blockers are extremely safe systemically and are useable for the treatment of systemic inflammation such as rheumatoid arthritis.

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Indication	Highest Phase	Country/Location	Organization	Date
Inflammation	Discovery	United States	The Texas A&M University System	-

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