Delving into the Latest Updates on IMP-361 with Synapse

Overview

Basic Info

Drug Type

Fusion protein

Synonyms

hLAG-3 + E7 fusion protein vaccine(Immutep)

Target

HPV E7

Mechanism

E7 modulators(Protein E7 modulators), Cells modulators

Therapeutic Areas

Infectious Diseases

Active Indication-

Inactive Indication

Human Papillomavirus Infection

Originator Organization

Immutep SAS

Active Organization-

Inactive Organization

Immutep SAS

Drug Highest PhasePendingDiscovery

First Approval Date-

Regulation-

OBJECTIVETo investigate the role and mechanism of microRNA-361 (miR-361) in apoptosis after myocardial ischemia-reperfusion (MI-R) injury.MATERIALS AND METHODSFor the in vivo experiments, the mice model of MI-R injury was established, and miR-361 was up-regulated via lentivirus with miR-298 overexpression. The expression of miR-361 and Bcl-2 associated X protein (BAX) were detected via Real Time-quantitative Polymerase Chain Reaction (qPCR) and Western blot (WB), respectively. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay was used to detect the apoptosis in myocardial tissues. MI-R injury was also simulated in vitro experiments, and the relationship between miR-361 and BAX was verified using Luciferase reporter vector. The effect of miR-361 on cardiomyocyte apoptosis was also detected at the cellular level.RESULTSIn vivo experiments showed that the miR-361 expression was down-regulated at MI-R injury area. The up-regulation of miR-361 significantly decreased the expression of BAX, reduced the myocardial apoptosis and inhibited the mitochondrial apoptosis pathway protein expression, including the cytochrome-c (Cyt-C) and cleaved caspase-3. In vitro experiments revealed that BAX was a target gene of miR-361 and further proved that miR-361 could inhibit the cytochrome-c and cleaved caspase-3 expression, as well as reduce the myocardial apoptosis through BAX.CONCLUSIONSMiR-361 could improve the myocardial apoptosis through the target gene BAX in MI-R injury.

100 Deals associated with IMP-361

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