Small-molecule pectin derived from Premna ligustroides Hemsl leaves

Ulcerative colitis (UC), a chronic idiopathic inflammatory bowel disease, is characterized by a prolonged and recurrent course. The classical Traditional Chinese Medicine (TCM) formula Simiao Pill (SMP) has demonstrated unique and widespread therapeutic effects on diarrhea and other intestinal inflammation. However, its material basis and potential mechanisms of action remain unclear.

This study aims to explore the mechanisms by which SMP alleviates UC, emphasizing its anti-inflammatory properties and its role in regulating gut microbiota.

The chemical composition of SMP was identified using UPLC-Q-TOF-MS/MS. Network pharmacology and molecular docking were applied to predict potential anti-UC targets and pathways. In vitro models in RAW264.7 cells and an in vivo mouse model induced by dextran sulfate sodium (DSS) were established to evaluate the potential mechanisms using molecular biology techniques. Additionally, gut microbiota changes were analyzed via 16S rRNA sequencing, and metabolic profiling was conducted using UPLC-Q-TOF-MS/MS.

SMP significantly improved UC symptoms by targeting 148 protein-related pathways, including TLR4/PI3K/Akt/NF-κB, a key inflammatory regulator. Molecular docking confirmed strong interactions between SMP compounds and targets. SMP reduced inflammation, restored gut barrier integrity, and modulated gut microbiota and lipid metabolism in UC mice.

SMP alleviates UC by regulating the TLR4/PI3K/Akt/NF-κB pathway, balancing gut microbiota, and improving lipid metabolism. These findings support SMP's potential as a UC treatment and warrant further clinical exploration.

Controlling enteric diseases of broilers is crucial. Among many additives, organic acids (OA) and their blends are gaining attention to combat diseases in the post-antibiotic era. The current study evaluated the potentials of short-chain fatty acids (SCFA) and medium-chain fatty acids (MCFA) blends and/or phenolic compounds on intestinal integrity, intestinal pH, caecal microbiota, and caecal SCFA profiles of broilers under necrotic enteritis (NE) challenge. The additives used were: (A) a blend of SCFA, MCFA, and a phenolic compound (SMP), (B) a blend of free and buffered SCFA with MCFA (SMF), and (C) a blend of free and buffered SCFA with a high concentration of MCFA (SHM). A total of 1,404 male parental chicks of Ross 308 broilers were randomly allocated to 78 floor pens on hatching day with 6 treatments replicated 13 times with 18 birds per pen. The treatments were: UCC, unchallenged control; CHC, challenged control; BAC, challenged group plus zinc bacitracin; SMP, challenged group plus additive SMP; SMF, challenged group plus additive SMF; SHM, challenged group plus additive SHM. Birds were challenged with field-strain Eimeria spp. on d 9 and Clostridium perfringens on d 14. Birds challenged with NE increased fluorescein isothiocyanate dextran (FITC-d) concentration in serum, reduced acetate and butyrate concentrations, and increased Bacteroides and C. perfringens load in the caeca (P < 0.05). Birds fed additives decreased FITC-d from gut to serum, reduced Bacteroides (d 16, P < 0.05) and numerically reduced C. perfringens load compared to CHC group. Birds fed additive SHM had higher concentrations of acetate and butyrate (d 21, P < 0.05) than CHC group but were not different from SMP and SMF groups. All the additives exhibited similar intestinal protection against NE compared to the BAC group indicated by FITC-d concentration in serum, acetate, propionate and butyrate concentrations in the caeca, and caecal bacterial loads except for the C. perfringens (P > 0.05). The SMP group had a higher load compared to BAC (P < 0.05). These findings suggest the promising effects of OA blends as alternatives to BAC to ameliorate the impact of NE challenge of broilers as indicated by improved intestinal health.