Auriclosene

Long-term use of indwelling urinary catheters can lead to urinary tract infections and loss of catheter patency due to encrustation and blockage. Encrustation of urinary catheters is due to formation of crystalline biofilms by urease-producing microorganisms such as Proteus mirabilis. An in vitro catheter biofilm model (CBM) was used to evaluate current methods for maintaining urinary catheter patency. We compared antimicrobial-coated urinary Foley catheters, with both available catheter irrigation solutions and investigational solutions containing NVC-422 (N,N-dichloro-2,2-dimethyltaurine; a novel broad-spectrum antimicrobial). Inoculation of the CBM reactor with 10(8) colony-forming units of P. mirabilis resulted in crystalline biofilm formation in catheters by 48 h and blockage of catheters within 5 days. Silver hydrogel or nitrofurazone-coated catheters did not extend the duration of catheter patency. Catheters irrigated daily with commercially available solutions such as 0.25 % acetic acid and isotonic saline blocked at the same rate as untreated catheters. Daily irrigations of catheters with 0.2 % NVC-422 in 10 mM acetate-buffered saline pH 4 or Renacidin maintained catheter patency throughout 10-day studies, but P. mirabilis colonization of the CBM remained. In contrast, 0.2 % NVC-422 in citrate buffer (6.6 % citric acid at pH 3.8) resulted in an irrigation solution that not only maintained catheter patency for 10 days but also completely eradicated the P. mirabilis biofilm within one treatment day. These data suggest that an irrigation solution containing the rapidly bactericidal antimicrobial NVC-422 in combination with citric acid to permeabilize crystalline biofilm may significantly enhance catheter patency versus other approved irrigation solutions and antimicrobial-coated catheters.

A number of mol. characteristics for the N-chlorotaurine structural analogs, amino acid chloramines and related compounds have been computed by the ab initio B3LYP/6-31G method.In particular, the characteristics were the Mulliken at. charges for the chloramine part and adjacent atoms.The quant. measure of the capabilities of the chloramines to react with the methionine sulfide group or the sulfhydryl group of reduced glutathione was their reaction rate constantsThe constants available in the literature and those found in our experiments have been depicted with an exponential equation of multiple correlations.In the case of the reaction with methionine, the high determination coefficient (R²) was obtained with five independent variables, which were the charges of available chlorine, nitrogen and carbon bonded with nitrogen, the bond length between the nitrogen and carbon atoms and the mol. weightThe equation was used to predict the rate constant values for the reactions between methionine and compounds containing available chlorine.The prediction showed that the structural analogs of N-chlorotaurine bearing two Me groups at β-carbon of taurine are remarkable for the low value of the rate constant under discussion.