Seocalcitol

The exceptional chemical and physical properties of nanomaterials have attracted growing interest in the field of bone tissue engineering. Single walled carbon nanotubes (SWCNTs) and MXenes are two types of unique nanomaterials that have shown promising potential for tissue engineering applications. In this study, we aimed to compare the differentiation capacity of MG-63 cells in the presence of carbon nanotubes (CNTs) and MXene nanomaterials. Methods: The cytotoxic effect of MXene and SWCNTs based nanocomposites was evaluated using the MTS assay. Also, the differentiation potential was evaluated using two models of stimulation. The first model was a co-stimulation of MG-63’s with EB1089 and an analogue of lysophosphatidic acid (LPA), (3S)-1-fluoro-3-hydroxy-4-(oleoyloxy)butyl-1-phosphonate (FHBP). The second model utilized [Formula: see text]-glycerophosphate ([Formula: see text]GP) and ascorbic acid (ASC). Results: MXene and SWCNTs showed no cytotoxic effect after 48[Formula: see text]h of culture. MXene could enhance the maturation of MG-63 in both models of differentiation compared to controls and SWCNTs. Conclusion: The incorporation of MXene into MG-63 cell culture suggests that it has a promising potential as a biomaterial for bone regeneration as it demonstrated improved osteoblast maturation compared to SWCNTs.

Many vitamin D analogs and sterols-related compounds have been identified and extracted from several plants including Solanaceae species. The aim of our study was to evaluate the antifungal potential of 15 vitamin D, D3 analogs, and some sterols-related compounds that have been detected in some plants along with 3 standard antifungal drugs, Isavuconazole, Fluconazole, and Voriconazole, on the Mucormycosis-sterol 14-alpha demethylase (CYP51) enzyme by in silico study.

In this work, the molecular simulation was used to prepare the specific enzyme and the antifungal profiles utilized for the molecular docking of the contemplated compounds and subsequent prediction for the mechanism of binding within the catalytic site of Sterol 14-Demethylase (CYP51) to speculate their binding affinities. Anti-fungal medications, Fluconazole and Voriconazole revealed drugs bound distant from the binding sites, but Isavuconazole bound close to the binding site. In this context, the 15 vitamin D and D3 compounds were somewhat seen in binding proximity, especially for Alfacalcidol, Eldecalcitol, and Inecalcitol.

Seocalcitol and Isavuconazole had the lowest binding energies and were the closest ones connected to the binding cavities with the best binding free energies equal −13.21 ± 0.24 and −15.29 ± 0.25, respectively.

Isavuconazole and Seocalcitol could potentially be further assessed to be adjuvantly used as drugs to inhibit the fungal activity by targeting CYP51, a significant target for anti-fungal as well as anti-protozoan drugs, and hence could be efficient against mucormycosis.