Early Enteral Administration of a Complex Lipid Emulsion Supplement Prevents Postnatal Deficits in Docosahexaenoic and Arachidonic Acids and Increases Tissue Accretion of Lipophilic Nutrients in Preterm Piglets

Q2 · MEDICINE

Article

Author: Ehling, Stefan ; Perides, George ; Martin, Camilia R. ; Freedman, Steven D. ; Sanchez‐Fernandez, Lady Leidy ; Pollack, Elizabeth ; Yakah, William ; Dao, Duy T. ; Obregon, Evelyn ; Liu, Yan ; DeMichele, Stephen ; Cluette‐Brown, Joanne ; Nedder, Arthur ; Anez‐Bustillos, Lorenzo ; Akinsulire, Olajumoke ; Kiefer, Savanna ; Singh, Pratibha ; Vurma, Mustafa ; Gordon, Douglas ; Bicak, Ece ; Gutierrez, Hilda V.

AbstractBackgroundPreterm delivery and current nutrition strategies result in deficiencies of critical long‐chain fatty acids (FAs) and lipophilic nutrients, increasing the risk of preterm morbidities. We sought to determine the efficacy of preventing postnatal deficits in FAs and lipophilic nutrients using an enteral concentrated lipid supplement in preterm piglets.MethodsPreterm piglets were fed a baseline diet devoid of arachidonic acid (AA) and docosahexaenoic acid (DHA) and randomized to enteral supplementation as follows: (1) Intralipid (IL), (2) complex lipid supplement 1 (CLS1) with an AA:DHA ratio of 0.25, or (3) CLS2 with an AA:DHA ratio of 1.2. On day 8, plasma and tissue levels of FAs and lipophilic nutrients were measured and ileum histology performed.ResultsPlasma DHA levels decreased in the IL group by day 2. In contrast, DHA increased by day 2 compared with birth levels in both CLS1 and CLS2 groups. The IL and CLS1 groups demonstrated a continued decline in AA levels during the 8‐day protocol, whereas AA levels in the CLS2 group on day 8 were comparable to birth levels. Preserving AA levels in the CLS2 group was associated with greater ileal villus height and muscular layer thickness. Lipophilic nutrients were effectively absorbed in plasma and tissues.ConclusionsEnteral administration of CLS1 and CLS2 demonstrated similar increases in DHA levels compared with birth levels. Only CLS2 maintained AA birth levels. Providing a concentrated complex lipid emulsion with an AA:DHA ratio > 1 is important in preventing postnatal AA deficits.

01 Jun 2013·Tissue Engineering Part C: MethodsQ4 · MEDICINE

Xenofree Enzymatic Products for the Isolation of Human Adipose-Derived Stromal/Stem Cells

Q4 · MEDICINE

Article

Author: Gomes, Manuela E. ; Reis, Rui L. ; Dias, Isabel R. ; Carvalho, Pedro P. ; Gimble, Jeffrey M.

Human adipose-derived stromal/stem cells (ASCs) are an abundant, readily available population of adult stem cells that reside in adipose tissue and that have a great potential utility for tissue engineering and regenerative medicine therapeutic applications. Several preclinical studies have shown that ASCs have therapeutic applicability, but a standardized isolation and expansion methodology for clinical cell therapy has yet to be established. ASC are typically isolated and expanded using reagents with xenogenic components and this may pose certain risks and safety issues, such as exposure to infectious agents and immune reactions, creating further obstacles to the translation of ASC-based therapies to clinical scenarios. The objective of this study was to determine the suitability and efficacy of various alternative enzymatic products, CLS1 (Worthington), CLSAFA (Worthington), NB4 (SERVA), and Liberase (Roche), for the digestion of adipose tissue and subsequent isolation of ASCs, assessing cell functionality concerning their proliferation and differentiation ability. Results show that there are no statistically significant differences on yield and proliferation of cells isolated after enzymatic digestion with any of the studied products. The differentiation potential of the cells was not affected, and cell surface marker expression was similar among all products. We concluded that clinical grade products can replace current research-grade products effectively in our cell isolation protocols without any negative effect in the yield or function of human ASCs.

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Indication	Highest Phase	Country/Location	Organization	Date
Obesity	Preclinical	HK	Aptorum Group Ltd.	-

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