OMT-002

Ischemia/reperfusion injury (IRI) causes cellular dysfunction and death in organs like the kidney, heart, and brain. It involves energy depletion during ischemia and oxidative stress, inflammation, and apoptosis during reperfusion. Kidney IRI often leads to acute kidney injury (AKI) in various clinical scenarios. The omentum, an adipose tissue with healing properties, has been used to treat injuries in different organs.

This study aimed to assess the omentum’s healing effects on reducing IRI’s adverse effects after renal ischemia in Wistar rats.

A total number of 36 male Wistar rats were used in a study on IRI-induced AKI. Rats were divided into 6 groups of normal kidneys wrapped with omentum “Sham-1” and “Sham-2,” ischemic kidney wrapped with omentum as “OMT-1” and “OMT-2,” and ischemic kidney without omentum as “Control-1” and “Control-2.” Ischemia was induced by clamping the left renal artery for 45 minutes. The omentum was transposed onto the injured kidney in “OMT” group. After sacrifice at weeks 4 and 8, kidney histology and blood samples were analyzed for kidney function markers.

On the first day after surgery, there was an immediate increase in creatinine and blood urea nitrogen (BUN) levels, which then decreased by day 28. Both OMT groups showed significantly lower levels of creatinine and BUN compared to Control groups on day 1, but after 28 days differences were not statistically significant. Histological analysis using H&E and Masson’s trichrome staining revealed significantly higher levels of inflammatory cell infiltration and hyperemia in the OMT groups. However, fibrosis and glomerular shrinkage were higher in the Control groups.

Using an omental flap significantly prevented fibrosis within the renal parenchyma, slow down the AKI progression, and potentially serving as a promising therapeutic strategy for kidney dysfunction.