LT-005

In this study, we identified and screened nine novel umami peptides derived from yellowfin tuna (Thunnus albacares) utilizing peptidomics combined with various machine learning based umami screening methodologies, including UMPred-FRL, TPDM, Umami-MRNN, and iUmami-SCM. The results of the multisensory evaluation indicated that SI-5 and VE-8 exhibited a high intensity of umami flavor, while AP-6, LD-5, and LT-5 effectively masked bitterness without compromising umami intensity. Furthermore, molecular docking techniques revealed interactions between the identified umami peptides and T1R1/T1R3 umami receptors as well as TAS2R14 bitter receptors, highlighting critical binding residues. Notably, LT-5 demonstrated a significant capacity to reduce quinine concentration, thereby enhancing the bitter-masking effect and elevating the bitterness threshold. These findings highlighted the potential of tuna-derived peptides as natural umami enhancers and bitterness inhibitors, providing valuable prospects for flavor improvement in food products.