WB001(Wayne Biotech)

Hydrogen peroxide (H₂O₂) is a physiological signaling molecule that plays essential roles in cellular processes but, when present in excess, induces oxidative stress and pathological damage. Aberrant mitochondrial production of H₂O₂ is closely associated with various diseases. In this study, we designed and synthesized a cyanine derivative-based near-infrared (NIR) fluorescent probe, WB-1. Ultraviolet-visible and fluorescence spectroscopic analyses revealed that WB-1 exhibits excellent selectivity and sensitivity for H₂O₂ detection, with a prominent fluorescence emission peak at 835 nm in the NIR region and a response time of 10 min. The probe exhibited a good linear response to H₂O₂ in the 0-20 μM range (R² = 0.987), achieving a detection limit as low as 63 nM. Cellular experiments demonstrated that WB-1 has low cytotoxicity, selectively targets mitochondria, and enables real-time tracking of transient changes in intracellular H₂O₂ levels. WB-1 was successfully applied in live-cell imaging and enabled real-time H₂O₂ visualization in zebrafish and mice. These capabilities position WB-1 as a valuable tool for providing critical insights into the pathogenesis of H₂O₂-related diseases.