Sulfamethazine

In this study, the absorption and fluorescence characteristics of sulfa drugs, specifically Sulfadiazine (SDZ), Sulfamerazine (SMZ), and Sulfamethazine (STZ), were examined across a pH range of 1-14. The absorption and fluorescence spectra of these sulfa drugs showed significant changes depending on the pH value. Analysis of their pH-dependent absorption and fluorescence properties indicated that these sulfa drugs could used to design molecular logic gates. The absorption and fluorescence behaviors at various wavelength maxima were utilized to design IMPLICATION and Improved-INHIBIT (I-INHIBIT) molecular logic gates.

Biosolids can provide a nutrient rich soil amendment, particularly for poor soils and semi-arid or drought-prone areas. However, there are concerns that sludge and biosolids could be a source of propagation and exposure to AMR determinants such as antibiotic resistant bacteria (ARB), and antibiotic resistance genes (ARGs). To inform risk assessment efforts, a systematic literature review was performed to build a comprehensive spreadsheet database of ARB and ARG concentrations in biosolids (and some sludges specified as intended for land application), along with 69 other quantitative and qualitative meta-data fields from 68 published studies describing sampling information and processing methods that can be used for modeling purposes. Mean ARG concentrations per gram in positive samples of biosolids ranged from -5.7 log₁₀(gene copies [gc]/g) to 12.92 log₁₀(gc/g) (with these range values reported per dry weight), and aqueous concentrations ranged from 0.9 log₁₀(gc/L) to 14.6 log₁₀(gc/L). Mean ARB concentrations per gram of biosolids ranged from 2.02 log₁₀ (colony forming units [CFU]/g) to 9.00 log₁₀ (CFU/g) (dry weight), and aqueous concentrations ranged from 3.23 log₁₀ (CFU/L) to 12.0 log₁₀ (CFU/L). ARG log removal values (LRVs) during sewage sludge stabilization were calculated from a meta-analysis of mean concentrations before and after stabilization from 31 studies, ranging from -2.05 to 5.52 logs. The classes of resistance most relevant for a risk assessment corresponded to sulfonamide (sul1 and sul2), tetracycline (tetZ, tetX, tetA and tetG), beta-lactam (bla_TEM), macrolide (ermB and ermF), aminoglycoside (strA and aac(6')-Ib-cr), and integron-associated (intI1). The resistance classes most relevant for ARB risk assessment included sulfonamides (sulfamethoxazole and sulfamethazine), cephalosporin (cephalothin and cefoxitin), penicillin (ampicillin), and ciprofloxin (ciprofloxacin). Considerations for exposure assessment are discussed to highlight risk assessment needs relating to antimicrobial resistance (AMR) associated with biosolids application. This study aids in prioritization of resources for reducing the spread of AMR within a One Health framework.