Recent reports have demonstrated that isolation of Salmonella spp. in atypical character is more prevalent. A total of 2,172 strains of Salmonella spp. were isolated from stool specimens examined in our laboratory from 1996 to 1999. Of these strains, 66(3.6%)were found to be H2S-neg. when cultured on SS, TSI, and SIMagar. These strains were identified as H2S-neg. Salmonella spp. by VITEK-GNI kit. H2S-neg. strains accounted for 0.8%, 0.296, 8.6%. and 2496 of total strains identified as Salmonella spp. in 1996, 1997, 1998, and 1999. resp. Of the 66 strains, 51 were available for serotyping and antibiogram testing. Forty-eight strains were identified as S. enteritidis and one of them was resistant to chloramphenicol. Two strains were S. Berta and both and were resistant to ampicillin and kanamycin. The remaining one strain was S. Litchfield and showed resistance to tetracycline. Of 44 H2S-neg. strains isolated in 1998,31 were recovered in Ibaraki area, suggesting that H2S-neg. strain was epidemic in the area.