The objective of this paper was to establish the quality standard of Guishen Pills.The qual. anal. of Cortex Eucornmiae and Fructus Lycii in Guishen Pills was carried out by TLC, and the content of loganin in Guishen Pills was determined by HPLC.The chromatog. spots of Cortex Eucommiae and Fructus Lycii were identified without the interference of neg. control.A linearity of loganin was obtained from 0.188 μg to 0.940 μg ,with a good correlation (r=0.999 8, n=6).The average recovery was 99.00% and the RSD was 1.02%.The qual. and quant. methods established in this study were simple, feasible, accurate and reproducible, and could be used as the quality control standard for Guishen Pills.