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Overview

An optimized protocol for obtaining full-length low-complexity cDNA libraries enriched for multiple-target genes using the RecA-method is described.This methods is validated by sequentially increasing the number of probes used in individual RecA reaction up to library-scale studies and showed that target cDNAs were efficiently enriched in each case.

Practical considerations for use of reef coral fluorescent proteins in mammalian cells: applications in fluorescence microscopy and flow cytometry

Author: Turpin, Pierre ; Haugwitz, Michael ; Kain, Steven R. ; Fang, Yu ; Dery, Olivier

A review.The discovery of fluorescent proteins from nonbioluminescent reef corals [reef coral fluorescent proteins (RCFPs)] has greatly expanded the panel of emission wavelengths available for in vivo analyses of cellular events and has opened the door to many new multicolor applications.These RCFP proteins differ not only in their excitation and emission spectra, but also in the time course of fluorescence development, relative brightness, and long-term expression.The unique properties of each RCFP, their expression in mammalian cells, and their use and detection in multiplex applications are described.

Functional Genomics

Plastic Microarrays: A Novel Array Support Combining the Benefi ts of Macro- and Microarrays

Article

Author: Faulstich, Konrad ; Granger, Claire ; Aivazachvili, Vissarion ; Chenchik, Alex ; Munishkin, Alexander

A protocol for printing long oligonucleotide (60-80 bases) arrays using a new type of array support, the BD Atlas Plastic Film, is presented.This novel array support is a clear, rigid sheet that requires no further treatment before printing.It combines the simplicity of nylon arrays with the high gene d. of glass arrays.

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