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A method was established for the determination of the intermediate contents of the sisomycin sulfate injection and alternative for content control in the production process of aminoglycoside antibiotics was found.The intermediate content of sisomycin sulfate injection was determined by polarimetry.Assay optical rotation used Na spectrum D line (589.3 nm) (tube length = 2 dm, sisomycin content per sample = 20 mg, column temperature = 20°).A methodol. survey pointed out there was a good linearity between 5 to 30 mg/mL^-1 of sisomicin sulfate and optical rotation units per mL.The corresponding average recovery was 100.1%, and RSD = 0.23% (n = 5).The method is simple, rapid and accurate.

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