Delving into the Latest Updates on Medical Center of Central Massachusetts with Synapse

Overview

Chilling induces shape changes in platelets from disks to spheres with abundant filopodia. Such changes were time-dependent and correlated well with the phosphorylation of 20-kDa myosin light chain (LC20). Both the shape changes and the phosphorylation were reversible. After the platelets had been chilled, myosin became incorporated into the Triton X-insoluble fraction. When the chilled platelets were immunocytochemically stained, anti-myosin antibody was localized with filamentous structures inside the filopodia. These results suggest that LC20 phosphorylation and subsequent interactions with actin filaments play a crucial role in the cold-induced changes in platelet shape and in the formation of filopodia.

01 Sep 1998·Cervical Spondylosis and Similar Disorders

The Development of the Surgical Approaches for Cervical Radiculopathy and Spondylotic Myelopathy

Author: SEYBOLD, E. A. ; DUNN, E. J.

01 May 1997·StrokeQ1 · MEDICINE

Synergistic Effects of Citicoline and MK-801 in Temporary Experimental Focal Ischemia in Rats

Q1 · MEDICINE

Article

Author: Fisher, Marc ; Li, Fuhai ; Tatlisumak, Turgut ; Locke, Kenneth W. ; Önal, M. Zülküf ; Sandage, Bobby W.

Background and Purpose Citicoline, a naturally occurring precursor of phosphatidylcholine, is neuroprotective and is currently being assessed in clinical trials. To evaluate potential synergistic neuroprotective effects of prolonged citicoline treatment and early N -methyl- d -aspartate (NMDA) antagonist therapy, suboptimal treatment regimens of citicoline and MK-801 were tested alone and in combination in a rat model of temporary focal ischemia. Methods Four groups of Sprague-Dawley rats (n=12 per group) underwent 90 minutes of temporary middle cerebral artery occlusion (MCAO) with the suture model. Animals were randomly and blindly assigned to one of four treatment groups: (1) saline, vehicle; (2) MK-801, 0.5 mg/kg IV bolus at 60 minutes after MCAO followed by saline 1 mL/kg IP daily for 7 days; (3) saline IV at 60 minutes after MCAO followed by citicoline 250 mg/kg IP daily for 7 days; or (4) both MK-801 and citicoline (daily for 7 days) active treatment. Triphenyltetrazolium chloride staining was used to assess postmortem infarct volume. Neurological scores were determined daily. Results Premature mortality between days 2 and 4 was 33.3% in group 1, 41.7% in groups 2 and 3, and 25.0% in group 4. Mean corrected infarct volume was significantly reduced in group 4 compared with the others (175.2±89.3 mm 3 in group 1, 179.1±78.5 mm 3 in group 2, 163.9±73.7 mm 3 in group 3, and 84.7±56.8 mm 3 in group 4 [ P <.02, ANOVA and P <.05, Scheffé’s test for group 1 versus group 4]). Mean infarct volume in animals dying prematurely was significantly ( P <.05, Student’s t test) larger in group 1 than those surviving for 7 days (247.2±89.5 versus 139.2±68.2 mm 3 ), but there was no significant difference in infarct volume in groups 2, 3, and 4 between animals dying prematurely and those surviving for 7 days. Conclusions These results demonstrate synergistic neuroprotective effects of citicoline and an NMDA antagonist in temporary experimental focal ischemia.

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Pipeline

Pipeline Snapshot as of 26 Dec 2024

The statistics for drugs in the Pipeline is the current organization and its subsidiaries are counted as organizations,Early Phase 1 is incorporated into Phase 1, Phase 1/2 is incorporated into phase 2, and phase 2/3 is incorporated into phase 3

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