The main goal of tissue engineering (TE) is supporting the regeneration of damaged tissues that are difficult to regenerate.The exptl. results of the preparation of semi-permeable membranes for cell cultures are presented.The effect of the PLA mol. weight and addition of pore precursors on the morphol. of the membranes was studied.The pore precursor of choice was polyvinylpyrrolidone (PVP).It was found that semi-permeable membranes for application in tissue engineering can be prepared with polylactides of mol. weight more significant than 37,000 g/mol.Moreover, it was observed that the growth of the mol. weight of the polymer, the porosity, the size of the pores, the Young modulus and maximum tensile increased.Addnl., to obtain a better morphol. of the membranes, PVP should be added to the polymeric solutionPos. growth of L929 fibroblast cells on the obtained scaffolds was shown.