Nucleotides, as important low mol. weight compounds, participate in a variety of regulatory and metabolic processes in organisms. 5'-nucleotides can be used as flavor enhancers to improve the sensory characteristics of foods because of their fresh enhancing effect.Dietary nucleoside plays an important role in maintaining functions of bone marrow hematopoietic cells, intestinal mucosa and brain, and has become natural functional food additives.At present, the large-scale production of nucleotides mainly adopts strategies such as chem. synthesis, fermentation, enzymic hydrolysis, and enzymic catalysis.In addition, sensitive and effective methods for the determination of nucleoside compounds are important to guarantee and improve food quality, chromatog. and electrophoresis have been applied.This review described the production and detection methods of nucleotide-based food additives, summarized the progress of their development and application in condiments and nutrient fortifiers, and give an outlook on future research directions and prospects, in the hope of providing a reference for further research of nucleotides and the development of related industries.