Limin Group Co., Ltd.

The high temperature modified covalent triazine framework (CTF-600) can exhibit the upmost specific capacitance of 458 F/g at 0.25 A/g, owing to the increased conductivity and suitable pore structure, accompanied by the inherent advantage of CTF.The corresponding sym. CTF-600//CTF-600 supercapacitor exhibits the energy d. of 8 Wh/kg, and excellent cycle stability such as no significant decline of capacitance retention after 10000 cycles.Therefore, the calcined CTF can be identified as a promising electrode material for supercapacitors.

Avermectin and its analogues are produced by the actinomycete Streptomyces avermitilis and are widely used in the field of animal health, agriculture, and human health. Here we have adopted a practical approach to successfully improve avermectin production in an industrial overproducer. Transcriptional levels of the wild-type strain and industrial overproducer in production cultures were monitored using microarray analysis. The avermectin biosynthetic genes, especially the pathway-specific regulatory gene, aveR , were up-regulated in the high-producing strain. The upstream promoter region of aveR was predicted and proved to be directly recognized by σhrdB in vitro. A mutant library of hrdB gene was constructed by error-prone PCR and selected by high-throughput screening. As a result of evolved hrdB expressed in the modified avermectin high-producing strain, 6.38 g/L of avermectin B1a was produced with over 50% yield improvement, in which the transcription level of aveR was significantly increased. The relevant residues were identified to center in the conserved regions. Engineering of the hrdB gene can not only elicit the overexpression of aveR but also allows for simultaneous transcription of many other genes. The results indicate that manipulating the key genes revealed by reverse engineering can effectively improve the yield of the target metabolites, providing a route to optimize production in these complex regulatory systems.

To accelerate the response rate of smart hydrogels to the environmental conditions, a novel pH-sensitive hydrogel microsphere with the controlled shapes and sizes were developed. Such monodispersed microspheres were synthesized via free radical polymerization under the protection of a multilayer stability system. The pH-responsibility of hydrogel microspheres was tested with the hydrogel bulk as a control. In vitro release studies were conducted in the simulated gastric fluid and intestinal juice with bovine serum albumin (BSA) as a model drug. The large specific surface areas endowed hydrogel microspheres a faster pH-responsibility than that of bulk ones. Therefore, such microspheres showed potential applications as the oral protein drugs carrier.