Shanghai JAKA Biotech Co Ltd.

Many researchers revealed that dermal fibroblast which mainly synthesis the collagen and elastin in dermis were affects by skin ageing, such as the proliferation rate and gene expression level.Oligopeptides were verified by many reports on anti-aging focus on collagen synthesis, but few literatures revealed the mol. mechanism of oligopeptides on skin aging.The potential anti-ageing effect and the mechanism of PTC, a new palmitoyl oligopeptide complex, were evaluated.MTT assay was adopted to detect the anti-aging effect of PTC in both human dermal fibroblast HDF-a and keratinocyte NHEK.The mechanism of PTC was detected further by full transcriptome anal. using RNA-sequencing method, and ELISA anal. of key proteins.Skin wrinkles were measured by VC98 in vivo.PTC showed pos. effect in cell proliferation and the biosynthesis of collagens, intergrins not only on fibroblasts, but also on keratinocytes.Moreover, many pathways were confirmed in the anti-aging effect of PTC, such as TGF-β1 pathway and ECM-receptor interaction.A cream contained 5.0% PTC showed significant improvement of skin wrinkles and roughness.So that, PTC has a potentially beneficial effect on the cell proliferation, the biosynthesis of ECM related proteins and the expression of many genes which are involved in cell cycle and secretory features to slow down skin aging.The mol. pathway maybe targets the TGF-β1 in both epidermis and dermis.

Photoaging or premature aging of skin is caused by a variety of factors, including hormone imbalance and exposure to UV radiation.Both UV light and polycyclic aromatic hydrocarbons in haze can cause skin aging and inflammation.Peach resin is a gum substance secreted from the bark of Rosaceae Prunus persica (L.) Batsch or Prunus davidiana (Carr.) Franch, which is beneficial to health.It also has the advantages of being natural and non-toxic, large in yield, and low in price.Peach gum is a kind of polysaccharide, which has the characteristics of swelling in water and slow release.At present, peach gum has been widely used in food and health care, but the basic research is still relatively weak.Peach gum is a natural cosmetic raw material with great potential for development and application.In this study, a Prunus persica (Peach) resin extract (PG) was prepared and its anti-aging effect was detected by several methods.The results suggested that, based on the model of fibroblast cell aging, PG could promote cell proliferation, collagen I expression and inhibit the activity of β-GAL in a dosage-dependent manner in the mass fraction range of 0.3% to 10%.In the cell damage model induced by UVA and BaP, PG at the mass fraction between 0.1% to 10% could inhibit the expression of inflammatory factors Prostaglandin E₂ and Matrix metallopeptidase 1.The results of clin. study showed that 5% and 10% PG had significantly increased skin tightening effect.In human clin. evaluation, PG at the mass fraction range of 2% to 10% could significantly increase skin moisture content, reduce transepidermal water loss, improve skin firmness, and reduce wrinkles.As a natural material with anti-aging effect, PG can be used in skin antiwrinkle products.

The anti-aging effect of Rejutime PTC, a peptide composition composed of hydrolyzed collagen and palmitoyl oligopeptide, was evaluated through different methods.The results show that PTC with a mass fraction of 0.125% to 0.5% could significantly promote cell proliferation and up-regulate the synthesis and secretion of type I, type III collagens and fibronectin after 24 to 72 h treatment on the aging model of fibroblasts (HDF-a).On the NHEK keratinocytes aging model, 0.063% and 0.25% of PTC also significantly promote cell proliferation after treatment for 24 to 72 h.For the four constituent proteins of the dermal epidermal junction (DEJ), namely laminin-5, integrin β1, type VII collagen and type IV collagen, PTC increase their synthesis and secretion at concentrations from 0.063% to 1%.PTC, as a new cosmetic additive, can play a role in delaying skin aging for skin epidermis, DEJ and dermis.