Effect of fiber milk on increasing bone mineral d. of ovariectomized rats was researched.One hundred and twenty SD rats aged 3 mo with 283 ± 15 g were randomly divided into sham operation group, control group, sample 1 group, sample 2 group, milk group and inulin group according to their body weightAfter feed for 12 w, body weight of 1st group was significantly lower than that of other groups, 2nd group was higher than other groups, and there was no difference in other groups.Bone mineral d. and bone calcium of femur in 2nd group were lower than those in 1st group, and the difference was significant (P < 0.01), which indicated osteoporosis model was successfully established.Bone mineral d. of femur in 3rd and 4th group was higher than that in 2nd, 5th, 6th group, and only 4th group had statistical difference.Bone mineral d. of distal part of femur in 4th group was higher than that in 2nd, 5th, 6th group, 3rd group was higher than 2nd, 6th group, 5th group was higher than 6th group, and the difference was significant.Bone strength in 3rd, 4th, 6th group was higher than 2nd group, and the difference was significant.Bone calcium in 4th, 6th group was higher than that in 2nd, 3rd group, and the difference was significant.Milk and inulin had additive effect on bone mineral d., and mix use is better than single use.Sample 1 and sample 2 could increase bone mineral d. in distal part of femur, and the effect was better than inulin.The effect of milk was poorer than sample 2, but was better than inulin.Sample 1, sample 2 and inulin could increase bone strength, and milk had no effect.Sample 2 and inulin could significantly increase bone calcium, and the effect of inulin on bone calcium had pos. correlation with dose.