Heavy loads of aerosols in the air have considerable health effects in individuals who suffer from chronic breathing difficulties. This problem is more acute in the Middle-East, where dust storms in winter and spring transverse from the neighboring deserts into dense populated areas. Discrimination between the dust types and association with their source can assist in assessment of the expected health effects. A method is introduced to characterize the properties of dense dust clouds with passive IR spectral measurements. First, we introduce a model based on the solution of the appropriate radiative transfer equations. Model predictions are presented and discussed. Actual field measurements of silicone-oil aerosol clouds with an IR spectro-radiometer are analyzed and compared with the theoretical model predictions. Silicone-oil aerosol clouds have been used instead of dust in our research, since they are composed of one compound in the form of spherical droplets and their release is easily controlled and repetitive. Both the theoretical model and the experimental results clearly show that discrimination between different dust types using IR spectral measurements is feasible. The dependence of this technique on measurement conditions, its limitations, and the future work needed for its practical application of this technique is discussed.

01 Nov 2001·Journal of Veterinary Diagnostic InvestigationQ4 · AGRICULTURAL & FORESTRY SCIENCE

Cultivation of Ehrlichia Canis in a Continuous BALB/C Mouse Macrophage Cell Culture Line

Q4 · AGRICULTURAL & FORESTRY SCIENCE

Article

Author: Strenger, Carmella ; Keysary, Avi ; Harrus, Shimon ; Waner, Trevor

This report describes the successful adaptation of the Israeli isolate of Ehrlichia canis on a continuous mouse macrophage cell line (J774.A1). Successful infection of the J774.A1 cells was first judged by the direct immunofluorescence antibody test using an anti- E. canis-IgG:FITC conjugate. A particular property of infected J774.A1 cells was the ability to reestablish after harvesting of the monolayer by scaping. Infected cells were used as antigen for immunofluorescence antibody tests (IFA), and the results compared well with those of DH82 cells. It was concluded that the J774.A1 continuous cell line could serve as an alternate propagation cell line for E. canis organisms.

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