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01 May 1990·American biotechnology laboratory
A technique for rapid protein and peptide analysis.
Article
Author: Nugent, K D
American biotechnology laboratory
A technique for rapid protein and peptide analysis.
American Biotechnology Laboratory
A technique for rapid protein and peptide analysis
Author: Nugent, Kerry D.
Although new columns have recently been introduced to allow rapid separation of protein mixtures, a great deal of care is required to optimize all of the instrumental parameters that will affect this technique.Since the important criterion in ultrafast protein anal. is the total anal. time from injection to injection with reproducible results, the column and HPLC system must be properly integrated.A dedicated analyzer was discussed (Ultrafast Microprotein Analyzer, UMA) that minimizes all of the system volumes and provides the other optimum system parameters to permit reproducible separations of complex protein mixtures in <5 min.Ultrafast protein anal. was extremely useful for recombinant protein process monitoring.By employing a UMA at line or directly online, process groups will be able to improve both yields and purity while saving anal. support and total process time.This technique is especially useful since the anal. can be run using a complementary mode of separation to that used in the process to enhance confidence in the results.This analyzer should prove useful for the rapid anal. of large numbers of protein samples.
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