Conduct time and temperature stability experiments on Nutritional Biomarkers (NBs) and inflammation markers extracted from ViveBio Dry Plasma Spots (DPS).Blood samples of 3 hematocrits (20, 30 and 45%) were created by combining purchased red blood cells (RBC) and sera.Blood samples of 35 μL were loaded onto ViveBio plasma separators.The underlying pads with plasma were stored at 45°, 23°, 4°, -20° and -70°C.Samples were tested on days 0, 1, 3, 7, 14, 28, 84 and 168.Ferritin, Retinol Binding Protein (RBP4), soluble Transferrin Receptors (sTfR), Thyroglobulin (Tg), α-acid Glycoprotein (AGP), and C-Reactive Protein (CRP) were measured using the Quansys Biosciences, Q-Plex Micronutrient Array (Q-Plex).Values measured in the liquid sera were used as reference values for the DPS.Red blood cell (RBC) folates and serum vitamin B12 were measured using a 96 well plate microbiol. assay (ALPCO-Immundiagnostik AG).25OH-Vitamin D3, Retinol, Methylmalonic acid (MMA) and Homocysteine (HCY) were measured using LC-MS/MS.The Q-Plex was compared at 3 independent laboratories using a group of 80 whole blood samples, non-spiked and spiked with AGP, CRP, HRP2 and sTfR.Liquid plasma and extracted plasma from ViveBio pads were generated and stored at -80°C four weeks prior to assay.Overall, the measured values for Ferritin, RBP4, Tg, AGP, CRP, 25OH-D, Retinol and MMA decrease with increasing storage temperature and length of storage.Ferritin and Tg exhibited the best stability at 16.8% and 8.0% over a 6 mo period.Vitamin B12 exhibited very poor stability declining to below the LOQ within day 3.Most of the NBs at 1 mo correlated well with liquid plasma values (r2 = 0.42 to 0.99) (AGP at 23°C, r2 = 0.428; at -70°C r2 = 0.990 Ferritin stored at 23°C, r2 = 0.854; at -70°C r2 = 0.992; Thyroglobulin at 23°C, r2 = 0.916; at -70°C r2 = 0.830).We have demonstrated that NBs can be analyzed in low volumes of DPS generated by the ViveBio plasma separator.The Q-Plex performed reproducibly on split samples among 3 laboratoriesThese tools offer great potential to improve nutritional assessment in population studies.Funding provided by the Bill and Melinda Gates foundation.