This study evaluated the ability of common herbal components in ergogenic dietary supplements to alter satellite cell activity in vitro. Herbal compounds studied were banaba leaf extract (1μg· ml−1, 10μg· ml−1, 100μg· ml−1, 1mg· ml−1), foenugreek seed extract (1μg· ml−1, 10μg· ml−1, 100μg· ml−1, 1mg· ml−1), andCystoseira canariensis extract (10pg· ml−1, 1ng· ml−1, 100ng· ml−1, 10μg· ml−1, 100μg· ml−1, 1mg· ml−1, 10mg· ml−1). Additionally, Ephedra sinenis extract andCitrus aurantium extract were reevaluated at lower levels (0.0000001%, 0.00001%, 0.001%). The effects of selected concentrations of these components were studied in combination with 0.1% creatine monohydrate, which we had previously determined possessed some capability to induce satellite cell differentiation. Ovine satellite cells were exposed to the four treatment levels of the herbal compounds for a specified amount of time and analyzed by counting mononucleated and multinucleated cells. None of the studied compounds, either alone or in comb...