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Physiol. fermentation parameters like different salts, buffers and pH were studied at shake flask level for Aspergillus sp.TL12 (3).Better lipase production observed in media containing salts MgSO4 7 H2O (20mM), CaCl2 (20mM) and Na2HPO4 (20mM).Best lipase yield identified in 50 mM glycine Hcl buffer at pH 6.The size of the inoculum found 1 % spore suspension (∼108 cells/mL) is an ideal for better lipase productionPhys. parameters of fermentation were studied.Temperature at 26 °C with 150 RPM shaking was found better.The duration of fermentation was found 72 h where maximum production of lipase was observedBy optimizing some of the physiol. parameters, lipase production has been increased to 1450 U/mL which is much better results at shake flask level as compared to several published results.

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