Alc. fatty liver disease (AFLD) is the earliest and the most common form of liver injury among the heavy alc. users and is also one of the precursor for severe forms of liver injury ranging from steatosis, inflammation and necrosis (steatohepatitis), to fibrosis and cirrhosis, and eventually hepatocellular carcinoma (HCC) in some cases. It is a worldwide public health issue and has been ever increasing with the increase of alc. consumption. Therefore, studies on the protection and prevention from AFLD have been actively carried out. Although, there has been a study conducted on the original Angelica gigas Nakai. powder that was introduced in the previous research, there is a coumarin-based lipid-soluble substance in the indexes of Angelica gigas Nakai. The decursin (DN) and decursinol angelate (DA), resp. DN and DA, the indicator elements of Angelica gigas Nakai, are structural isomers which are only the structure of the side chain region and difficult to sep. due to their similar phys. properties. DN and DA were selectively synthesized from decursinol, which was hydrolyzed in the extract of Angelica gigas Nakai. The synthesized compounds namely DN, DA, DL and mixture (DN and DA) was administered to C57BL / 6 mice (N=5/group) for 12 wk with 25% EtOH. Serum biochem. parameters AST, ALT and TG were determined, and significant difference was observed for AST and TG between alc. treated group and compound treated groups. H&E staining anal. also validated the reduction of lipid droplets in liver tissue samples from DN, DA and mixture as compared with alc. treated group. Immunohistochem. staining anal. on liver tissue for TNF-α showed reduced levels on DN, decursinol and mixture Finally, Cytochrome P 450 2E1 (CYP2E1) which plays a critical role in ROS generation was analyzed through Western blotting. Results indicated the compound treated groups had relatively lower expression of CYP2E1 compared with the alc. treated group. Altogether, these results revealed coumarin-based lipid soluble substance extracted from Angelica gigas Nakai ameliorated the liver damage caused by ethanol abuse on C57BL/6 mice comparing with the normal control group.