The content of puerarin in Ganmaozhike was established.The condition was as follows: C18 (250 mm × 4.6 mm, 5 μm), methanol-0.3% phosphoric acid (27:73) as flow phrase, detected wavelength: 250 nm, flow rate: 1.0 mL/min-1.The puerarin was in the range of 4.41-141.06 μg/mL-1 (n = 6), and it had good linear relationship (r = 0.9998).The average recovery was 97.9%, RSD = 0.96%.The method is quick, sensitive, and convenient, which can be used in quality control of Ganmaozhike granule.