A single-arm, single-center, open-labeled study will be conducted with an aim to investigate the feasibility, safety, and efficacy of the personalized vaccine for patients with recurrent malignant glioma.

Start Date15 Jul 2021

Sponsor / Collaborator

Xuanwu Hospital Capital Medical University

[+1]

100 Clinical Results associated with Beijing NeoAntigen Biotechnology Co., Ltd.

0 Patents (Medical) associated with Beijing NeoAntigen Biotechnology Co., Ltd.

Literatures (Medical) associated with Beijing NeoAntigen Biotechnology Co., Ltd.

21 Mar 2022·Nucleic Acids Research

Grouped-seq for integrated phenotypic and transcriptomic screening of patient-derived tumor organoids

Article

Author: Sun, Tao ; Wu, Yushuai ; Liu, Chang ; Chen, Xiaojie ; Li, Yaqian ; Zhao, Tian ; Tang, Decong ; Li, Kaiyi ; Chen, Xiaofang ; Liu, Peng ; Dong, Chunhui

AbstractPatient-derived tumor organoids (PDOs) have emerged as a reliable in vitro model for drug discovery. However, RNA sequencing-based analysis of PDOs treated with drugs has not been realized in a high-throughput format due to the limited quantity of organoids. Here, we translated a newly developed pooled RNA-seq methodology onto a superhydrophobic microwell array chip to realize an assay of genome-wide RNA output unified with phenotypic data (Grouped-seq). Over 10-fold reduction of sample and reagent consumption together with a new ligation-based barcode synthesis method lowers the cost to ∼$2 per RNA-seq sample. Patient-derived colorectal cancer (CRC) organoids with a number of 10 organoids per microwell were treated with four anti-CRC drugs across eight doses and analyzed by the Grouped-seq. Using a phenotype-assisted pathway enrichment analysis (PAPEA) method, the mechanism of actions of the drugs were correctly derived, illustrating the great potential of Grouped-seq for pharmacological screening with tumor organoids.

01 Jan 2020·Journal of Assisted Reproduction and GeneticsQ3 · MEDICINE

Transcriptome sequencing of endometrium revealed alterations in mRNAs and lncRNAs after ovarian stimulation

Q3 · MEDICINE

Article

Author: Bai, Xueyan ; Li, Yuan ; Li, Lingxiu ; Wang, Peng ; Zou, Binbin ; Liu, Shan

RESEARCH QUESTIONUsing RNA-sequencing analysis, we investigated the relationship between ovarian stimulation and endometrial transcriptome profiles during the window of implantation (WOI) to identify candidate predictive factors for the WOI and to optimize timing for embryo transfer.METHODSTwelve women with normal basal hormone levels and regular ovulation were randomly assigned into three groups based on sampling time: late-proliferate phase (P group), and receptive phase in natural cycles (LH+7, N group) and stimulated cycles (hCG+7, S group). Transcriptome profiles of mRNAs and long non-coding RNAs (lncRNAs) were then compared among the three groups. Validation was performed using real-time qPCR.RESULTSComparison of transcriptome profiles between the natural and stimulated endometrium revealed 173 differentially expressed genes (DEGs), with a > 2-fold change (FC) and p < 0.05, under the influence of supraphysiological estradiol (E₂) induced by ovarian stimulation. By clustering and KEGG pathway analysis, molecules and pathways associated with endometrial receptivity were identified. Of the 39 DEGs common to the three groups, eight genes were validated using real-time PCR. ESR1, MMP10, and HPSE were previously reported to be associated with endometrial receptivity. In addition, three novel genes (IL13RA2, ZCCHC12, SRARP) and two lncRNAs (LINC01060, LINC01104) were new potential endometrial receptivity-related markers.CONCLUSIONUsing mRNA and lncRNA sequencing, we found that supraphysiological E₂ levels from ovarian stimulation had a marked impact upon endometrial transcriptome profiles and may result in a shift of the WOI. The precise mechanisms underlying the supraphysiological hormone-induced shift of the WOI require further research.REGISTRATION NUMBERChiCTR180001453.

01 Sep 2018·Molecular & Cellular Proteomics

Cell Lysate Microarray for Mapping the Network of Genetic Regulators for Histone Marks

Article

Author: Huang, Jin-Guo ; Qi, Huan ; Zhou, Yi-Ming ; Liu, Cheng-Xi ; Hou, Sha ; Jiang, Shuangying ; Jiang, He-Wei ; Tao, Sheng-Ce ; Cheng, Li ; Wang, Jing-Fang ; Chen, Zi-Qing ; Czajkowsky, Daniel M ; Sun, Yang-Yang ; Dai, Junbiao

Proteins, as the major executer for cell progresses and functions, its abundance and the level of post-translational modifications, are tightly monitored by regulators. Genetic perturbation could help us to understand the relationships between genes and protein functions. Herein, to explore the impact of the genome-wide interruption on certain protein, we developed a cell lysate microarray on kilo-conditions (CLICK) with 4837 knockout (YKO) and 322 temperature-sensitive (ts) mutant strains of yeast (Saccharomyces cerevisiae). Taking histone marks as examples, a general workflow was established for the global identification of upstream regulators. Through a single CLICK array test, we obtained a series of regulators for H3K4me3, which covers most of the known regulators in S. cerevisiae We also noted that several group of proteins are involved in negatively regulation of H3K4me3. Further, we discovered that Cab4p and Cab5p, two key enzymes of CoA biosynthesis, play central roles in histone acylation. Because of its general applicability, CLICK array could be easily adopted to rapid and global identification of upstream protein/enzyme(s) that regulate/modify the level of a protein or the posttranslational modification of a non-histone protein.

100 Deals associated with Beijing NeoAntigen Biotechnology Co., Ltd.

100 Translational Medicine associated with Beijing NeoAntigen Biotechnology Co., Ltd.

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