The difficulty of correlating results of replicate disk separations, routinely encountered, because of variations from one gel-electrophoresis cylinder to another, was eliminated by applying multiple samples to a single vertical acrylamide-gel slab, so that individual components of many samples processed together on the same gel slab could be traced across sample boundaries with high reliability, employing the principle of continuity.Direct cooling of the gel slab located between 2 parallel H2O-cooled plates significantly improved the reproducibility from run to run, the cell requiring no gaskets or dialyzing membranes to retain the gel during polymerization, and being dismantled to remove the gel slab at the end of the electrophoresis run.Application of the sample in solution avoided the artefacts and loss of sample components which occurred when the sample was polymerized into a sample gel.Diluting the sample solutions with polymerized acrylamide solution (omitting cross-linking agent) dialyzed against the appropriate buffer until free of catalyst residues enabled the fulfillment of the requirements that the d. of the sample solution must be greater than that of the electrode buffer, and that the sample lots, in order to maintain a straight buffer front, must be filled to the top of the gel with a solution of approx. the same buffer composition and pH, and with nearly the same viscosity effect, as the spacer gel.An example is given of the successful application of different staining solutions, in sequence, to the entire slab for the demonstration of serum-protein patterns, the solutions involving stains for lactic dehydrogenase (tetrazolium reaction), haptoglobins (peroxidase reaction), and general protein (Light Green SF).

16 Sep 1966·ScienceQ1 · CROSS-FIELD

Electrochromatography with Reversing Electrophoretic Field

Q1 · CROSS-FIELD

Article

Author: Raymond, Samuel ; Broome, John

A protein mixture can be separated in a flow column by application of a transverse electrophoretic field to carry faster-migrating components of the mixture into a fixed gel bed at one side of the column, so that slower components move along in a buffer stream. The field is then reversed to return the faster-moving components to the buffer stream. The cycle is repeated many times to complete the separation.

01 Jan 1966·Separation Science

The Recovery of Serum Proteins by Elution Convection After Gel Electrophoresis

Author: Broome, J. ; Jordan, Esther M.

The recovery of serum proteins by elution convection was determinedDepending on the precise conditions, the recoveries ranged from 40% to nearly quant.The resolution was considered sufficient to justify this technique as a useful single-step fractionation procedure.Maximum total recovery by elution convection of serum proteins following gel electrophoresis was achieved in a system containing 21 mg. total protein, using Tris buffer, pH 8.4, and 300 v./hr. for elution, a cooling tank, and liquid sample, yielding 101% recovery, and in a system containing 36 mg. total protein, using glycine buffer, pH 9.2, and 230 v./hr. for elution, a cooling tank, and liquid sample, yielding 96% recovery.15 references.

100 Deals associated with Current, Inc.

100 Translational Medicine associated with Current, Inc.

Corporation Tree

Boost your research with our corporation tree data.

view full example data

Pipeline

Pipeline Snapshot as of 01 Jul 2025

No data posted

Deal

Boost your decision using our deal data.

view full example data

Translational Medicine

Boost your research with our translational medicine data.

view full example data

Profit

Explore the financial positions of over 360K organizations with Synapse.

view full example data

Grant & Funding(NIH)

Access more than 2 million grant and funding information to elevate your research journey.

view full example data

Investment

Gain insights on the latest company investments from start-ups to established corporations.

view full example data

Financing

Unearth financing trends to validate and advance investment opportunities.

view full example data

AI Agents Built for Biopharma Breakthroughs

Accelerate discovery. Empower decisions. Transform outcomes.

Get started for free today!

Accelerate Strategic R&D decision making with Synapse, PatSnap’s AI-powered Connected Innovation Intelligence Platform Built for Life Sciences Professionals.

Start your data trial now!

Synapse data is also accessible to external entities via APIs or data packages. Empower better decisions with the latest in pharmaceutical intelligence.

Bio

Bio Sequences Search & Analysis

Chemical

Chemical Structures Search & Analysis

Current, Inc.

Overview

Related

Corporation Tree

Pipeline

Pipeline Snapshot as of 01 Jul 2025

Deal

Translational Medicine

Profit

Grant & Funding(NIH)

Investment

Financing