Devansh Lab Werks, Inc.

Broadly targeted real-time PCR techniques for early diagnosis of viral infection by detection of virus nucleic acid (DNA and RNA) and virus antibodies (IgM) were developed.The developed QRT-PCR detected EV RNA from samples which contain a few copies of RNA (1-10 gene equivalent per PCR reaction) and from a broad variety of EVs in reference panels.Our results indicate that QPIA achieves a rapid diagnosis of enterovirus infection.The combination of QRT-PCR and QPIA detected 40/43 (93%) of aseptic meningitis cases from which both serum and CSF were available.A broadly targeted, simple, single tube real-time degenerated quant. PCR (QPCR) technique for detection of JCV, BKV and SV40 DNA was developed.At least down to 10-100 gene equivalent of target DNA per PCR reaction were detected. The QPCR is simple to perform and should be valuable for diagnosis of polyoma virus infection.The previous results regarding the frequencies of IgM antibodies against EVs in newly diagnosed T1D children, siblings and controls were confirmed by using viral antigen isolated from T1D cases at onset (T1D-EV-QPIA).

This study explores the preparation and assessment of microspheres formulated from natural gums, aiming for effectiveness, biodegradability, availability, and cost efficiency, using Lamivudine as the model drug.Lamivudine, with a biol. half-life of 4 to 6 h and 86% of bioavailability, is known for treating HIV and also for chronic Hepatitis B.The microspheres are developed via solvent evaporation technique employing xanthan gum and guar gum.Compatibility was confirmed through FTIR anal., showing no significant changes in characteristic peaks of Lamivudine and excipients across formulations.Particle size variation, surface morphol. of particle, % of yield, % of drug entrapment efficiency, in-vitro drug release profiles, release kinetics, and stability were evaluated.The microspheres were pale yellow and free-flowing, with micrometric properties meeting standards for good flow.SEM (SEM) revealed spherical shapes ranging from 100μm to 200μm.In-vitro drug release studies indicated a reduction in release rate with increasing xanthan gum concentration, following zero-order kinetics, suggesting diffusion-controlled release and stability.In conclusion, microspheres utilizing xanthan gum present a viable method for achieving controlled release of Lamivudine, potentially enhancing bioavailability and reducing dosing frequency.

Chronic kidney disease is an irreversible condition that progresses relentlessly leading sooner or later to the end stage renal failure.The diagnosis of this stage can be achieved by eliciting the history carefully, discovering co-morbid factors, utilizing imaging techniques, interpreting histol. material and placing this in the context of probability derived from epidemiol. data.Screening of high-risk individuals- those with hypertension, diabetes mellitus, cardiovascular and other risk factors, lifestyle modification, phys. exercise, abstinence from smoking will retard the progression to ESRD.This will help in bringing down the huge burden due to the mismatch between demand and availability of resources for renal replacement therapy in developing countries like India, especially for patients belonging to lower socioeconomic group.The assessment of the clin. profile of these patients showed the most common etiol. as diabetes mellitus (36.9%).Hypertension being a cause and a complication of CKD was present in 64.6% of patients.Early detection and effective management of these illnesses can delay the onset, progression of CKD and subsequent morbidity and the requirement of renal replacement therapy, if any.Another manageable condition obstructive uropathy found in 10% of these patients, if treated at an early stage prevents progression to irreversible kidney damage.Cardiovascular disease in chronic kidney disease is more common in the presence of Diabetes mellitus, Hypertension, hypernatremia, increased kidney size, hypo albuminuria in patients with anemia, cardiovascular disease was more common when Hb levels were less than 5g/dL.Cardiovascular diseases as a morbidity were identified in 28.5% patients.Being the leading cause of mortality in CKD it would be imperative to monitor patients for this morbidity.Cardiac structural, as well as functional abnormalities, are common in patients with ESRD, more so in those with hypertension and anemia.LVH is the commonest cardiac abnormality in ESRD patients, followed by diastolic dysfunction.Both conditions are more marked in hypertensive patients and anemic patients.LVH has got prognostic implications, because this group of ESRD patients have a propensity of diastolic dysfunction or sudden cardiac death.