The author aimed to determine comprehensive age and sex 99th percentiles based on cardiovascular disease patient demographics for high-sensitivity cardiac troponin I assay. The author defined healthy patients, comprising the reference population by having blood concentrations within the reference limits of the following 12 biomarkers for cardiovascular, endocrine, renal and liver disease: N-terminal pro-brain natriuretic peptide (normal: <125or<450pg/mL for<75 and >=75 years, resp.), tumor necrosis factor-α (normal: <3-0 pg/mL, cutoff set at the 95th percentile from 279 self-reported healthy donors), TSH (normal: 0.27-4.20 /fmIU/mL), Hb A1c (normal: >=5.6%), alanine aminotransferase (normal: 5-33 or 5-41 U/L for women and men, resp.), total bilirubin (normal: <1.3 mg/dL), alk. phosphatase (normal: 35-104 or 40-129 U/L for women and men, resp.), calcium (normal:8.6-10.2 mg/dL), creatinine (estimated glomerular filtration rate not available, normal: 0.5-0.9 or 0.7-1.2 mg/dL for women and men, resp.), sodium (normal: 136-145 mmol/L), potassium (normal: 3.5-5.1 mmol/L), and carbon dioxide (normal: 22-29 mmol/L). After defining the apparently healthy population, outliers were considered using the Tukey method, but this method was too restrictive because of the highly right-skewed cTnI distribution. Therefore, the Vanderloo Extreme Values and Reed outlier methods were sep. applied to the cTnI results. The final reference population consisted of 1645 unique patients and was separated by age and sex. The author concluded that final 99th percentiles for subgroups of women <50 years, women >=50 years, men <50 years, and men >=50 years were 4.1, 5.9, 5.8, and 6.1 ng/L, resp., using Vanderloo Extreme Values outlier method.